Coexpression of Pdx1 and betacellulin in mesenchymal stem cells could   promote the differentiation of nestin-positive epithelium-like   progenitors and pancreatic islet-like spheroids

doi:10.1089/scd.2008.0060

CORC > 北京大学 > 生命科学学院

	Coexpression of Pdx1 and betacellulin in mesenchymal stem cells could promote the differentiation of nestin-positive epithelium-like progenitors and pancreatic islet-like spheroids
	Li, Lisha ; Li, Furong ; Qi, Hui ; Feng, Gao ; Yuan, Kehu ; Deng, Hongkui ; Zhou, Hanxin
刊名	干细胞与发育
	2008
关键词	INSULIN-PRODUCING CELLS IN-VITRO MULTIPOTENTIAL NESTIN PRECURSOR CELLS TRANSPLANTATION EXPRESSION GROWTH MANIPULATION ENDOCRINE SECRETION
DOI	10.1089/scd.2008.0060
英文摘要	Mesenchymal stem cells (MSCs) have already been proved to be multipotent. Our goal was to evaluate the differentiating ability of rat MSCs into insulin-secreting cells in vitro to cure diabetes resulting from abnormal function of pancreatic islets. MSCs were identified by Euorescence-activated cell sorting (FACS). Pdx1 is a transcription factor involved in the early endocrine development. Betacellulin (BTC) is a growth factor involved in beta-cell maturation. MSCs were transfected with plasmids carrying rat Pdx1 and BTC genes. Coexpression of Pdx1 and BTC significantly increased the number of nestin-positive epithelium-like progenitors and islet-like spheroids which differentiated from MSCs. In Pdx1- and BTC-expressed (Pdx1(+) + BTC+) MSCs, insulin and Glut-2 mRNA levels significantly rose. The number of islet-like cells was also evidently augmented. In response to glucose, Pdx1(+) + BTC+ MSCs released insulin and C-peptide. It is concluded that genetic manipulation of transcription factor Pdx1 and growth factor BTC in combination with appropriate differentiating culture could induce MSCs into the pancreatic lineage in vitro and produce islet-like spheroids that could secrete increased levels of insulin in response to glucose.; Cell & Tissue Engineering; Hematology; Medicine, Research & Experimental; Transplantation; SCI(E); PubMed; 18; ARTICLE; 4; 815-823; 17
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/345237]
专题	生命科学学院
推荐引用方式 GB/T 7714	Li, Lisha,Li, Furong,Qi, Hui,et al. Coexpression of Pdx1 and betacellulin in mesenchymal stem cells could promote the differentiation of nestin-positive epithelium-like progenitors and pancreatic islet-like spheroids[J]. 干细胞与发育,2008.
APA	Li, Lisha.,Li, Furong.,Qi, Hui.,Feng, Gao.,Yuan, Kehu.,...&Zhou, Hanxin.(2008).Coexpression of Pdx1 and betacellulin in mesenchymal stem cells could promote the differentiation of nestin-positive epithelium-like progenitors and pancreatic islet-like spheroids.干细胞与发育.
MLA	Li, Lisha,et al."Coexpression of Pdx1 and betacellulin in mesenchymal stem cells could promote the differentiation of nestin-positive epithelium-like progenitors and pancreatic islet-like spheroids".干细胞与发育 (2008).