Sequential phosphorylation of Nedd1 by Cdk1 and Plk1 is required for   targeting of the gamma TuRC to the centrosome

doi:10.1242/jcs.042747

CORC > 北京大学 > 生命科学学院

	Sequential phosphorylation of Nedd1 by Cdk1 and Plk1 is required for targeting of the gamma TuRC to the centrosome
	Zhang, Xiaoyan ; Chen, Qiang ; Feng, Jia ; Hou, Junjie ; Yang, Fuquan ; Liu, Junjun ; Jiang, Qing ; Zhang, Chuanmao
刊名	journal of cell science
	2009
关键词	Cdk1 Nedd1 Plk1 Centrosome Phosphorylation gamma TuRC TUBULIN RING COMPLEX POLO-LIKE KINASE MICROTUBULE NUCLEATION MITOTIC KINASE CYCLIN B1-CDK1 BOX DOMAIN PROTEIN SPINDLE ORGANIZATION MITOSIS
DOI	10.1242/jcs.042747
英文摘要	Nedd1 is a new member of the gamma-tubulin ring complex (gamma TuRC) and targets the gamma TuRC to the centrosomes for microtubule nucleation and spindle assembly in mitosis. Although its role is known, its functional regulation mechanism remains unclear. Here we report that the function of Nedd1 is regulated by Cdk1 and Plk1. During mitosis, Nedd1 is firstly phosphorylated at T550 by Cdk1, which creates a binding site for the polo-box domain of Plk1. Then, Nedd1 is further phosphorylated by Plk1 at four sites: T382, S397, S637 and S426. The sequential phosphorylation of Nedd1 by Cdk1 and Plk1 promotes its interaction with gamma-tubulin for targeting the gamma TuRC to the centrosome and is important for spindle formation. Knockdown of Plk1 by RNAi decreases Nedd1 phosphorylation and attenuates Nedd1 accumulation at the spindle pole and subsequent gamma-tubulin recruitment at the spindle pole for microtubule nucleation. Taken together, we propose that the sequential phosphorylation of Nedd1 by Cdk1 and Plk1 plays a pivotal role in targeting gamma TuRC to the centrosome by promoting the interaction of Nedd1 with the gamma TuRC component gamma-tubulin, during mitosis.; Cell Biology; SCI(E); PubMed; 39; ARTICLE; 13; 2240-2251; 122
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/344961]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zhang, Xiaoyan,Chen, Qiang,Feng, Jia,et al. Sequential phosphorylation of Nedd1 by Cdk1 and Plk1 is required for targeting of the gamma TuRC to the centrosome[J]. journal of cell science,2009.
APA	Zhang, Xiaoyan.,Chen, Qiang.,Feng, Jia.,Hou, Junjie.,Yang, Fuquan.,...&Zhang, Chuanmao.(2009).Sequential phosphorylation of Nedd1 by Cdk1 and Plk1 is required for targeting of the gamma TuRC to the centrosome.journal of cell science.
MLA	Zhang, Xiaoyan,et al."Sequential phosphorylation of Nedd1 by Cdk1 and Plk1 is required for targeting of the gamma TuRC to the centrosome".journal of cell science (2009).