Characterization and Prediction of Lysine (K)-Acetyl-Transferase   Specific Acetylation Sites

doi:10.1074/mcp.M111.011080

CORC > 北京大学 > 生命科学学院

	Characterization and Prediction of Lysine (K)-Acetyl-Transferase Specific Acetylation Sites
	Li, Tingting ; Du, Yipeng ; Wang, Likun ; Huang, Lei ; Li, Wenlin ; Lu, Ming ; Zhang, Xuegong ; Zhu, Wei-Guo
刊名	molecular cellular proteomics
	2012
关键词	LARGE GENE LISTS NONHISTONE PROTEINS HISTONE ACETYLTRANSFERASES IN-VITRO EXPRESSION CHROMATIN P300 P53 PHOSPHORYLATION INDUCTION
DOI	10.1074/mcp.M111.011080
英文摘要	Lysine acetylation is a well-studied post-translational modification on both histone and nonhistone proteins. More than 2000 acetylated proteins and 4000 lysine acetylation sites have been identified by large scale mass spectrometry or traditional experimental methods. Although over 20 lysine (K)-acetyl-transferases (KATs) have been characterized, which KAT is responsible for a given protein or lysine site acetylation is mostly unknown. In this work, we collected KAT-specific acetylation sites manually and analyzed sequence features surrounding the acetylated lysine of substrates from three main KAT families (CBP/p300, GCN5/PCAF, and the MYST family). We found that each of the three KAT families acetylates lysines with different sequence features. Based on these differences, we developed a computer program, Acetylation Set Enrichment Based method to predict which KAT-families are responsible for acetylation of a given protein or lysine site. Finally, we evaluated the efficiency of our method, and experimentally detected four proteins that were predicted to be acetylated by two KAT families when one representative member of the KAT family is over expressed. We conclude that our approach, combined with more traditional experimental methods, may be useful for identifying KAT families responsible for acetylated substrates proteome-wide. Molecular & Cellular Proteomics 11: 10.1074/mcp.M111.011080, 1-9, 2012.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000299106200010&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemical Research Methods; SCI(E); PubMed; 4; ARTICLE; 1; M111.011080; 11
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/343747]
专题	生命科学学院
推荐引用方式 GB/T 7714	Li, Tingting,Du, Yipeng,Wang, Likun,et al. Characterization and Prediction of Lysine (K)-Acetyl-Transferase Specific Acetylation Sites[J]. molecular cellular proteomics,2012.
APA	Li, Tingting.,Du, Yipeng.,Wang, Likun.,Huang, Lei.,Li, Wenlin.,...&Zhu, Wei-Guo.(2012).Characterization and Prediction of Lysine (K)-Acetyl-Transferase Specific Acetylation Sites.molecular cellular proteomics.
MLA	Li, Tingting,et al."Characterization and Prediction of Lysine (K)-Acetyl-Transferase Specific Acetylation Sites".molecular cellular proteomics (2012).