Purification and proteomic analysis of liver membrane skeletons

doi:10.1007/978-1-61779-959-4_7

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	Purification and proteomic analysis of liver membrane skeletons
	He Jintang ; Liu Yashu ; Wang Qingsong ; Ji Jianguo
刊名	methods in molecular biology clifton n j
	2012
DOI	10.1007/978-1-61779-959-4_7
英文摘要	The detergent-resistant membrane skeletons play a critical role in cell shaping and signaling. The focus of the methods described in this chapter is first on the preparation of membrane skeletons from liver by multistep sucrose density gradient centrifugation, and then on the analysis of the protein components of membrane skeletons using proteomics techniques. Two proteomic analysis strategies are described. In the first strategy, membrane skeleton proteins are separated by two-dimensional gel electrophoresis and identified by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry. In the other strategy, proteins are separated by SDS-PAGE and identified by liquid chromatography-electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry. The methods facilitate the understanding of the structure of membrane skeletons.; PubMed; 0; 97-111; 909
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/343183]
专题	生命科学学院
推荐引用方式 GB/T 7714	He Jintang,Liu Yashu,Wang Qingsong,et al. Purification and proteomic analysis of liver membrane skeletons[J]. methods in molecular biology clifton n j,2012.
APA	He Jintang,Liu Yashu,Wang Qingsong,&Ji Jianguo.(2012).Purification and proteomic analysis of liver membrane skeletons.methods in molecular biology clifton n j.
MLA	He Jintang,et al."Purification and proteomic analysis of liver membrane skeletons".methods in molecular biology clifton n j (2012).