Chromosomal deletions and inversions mediated by TALENs and CRISPR/Cas   in zebrafish

doi:10.1093/nar/gkt464

CORC > 北京大学 > 生命科学学院

	Chromosomal deletions and inversions mediated by TALENs and CRISPR/Cas in zebrafish
	Xiao, An ; Wang, Zhanxiang ; Hu, Yingying ; Wu, Yingdan ; Luo, Zhou ; Yang, Zhipeng ; Zu, Yao ; Li, Wenyuan ; Huang, Peng ; Tong, Xiangjun ; Zhu, Zuoyan ; Lin, Shuo ; Zhang, Bo
刊名	核酸研究
	2013
关键词	ZINC-FINGER NUCLEASES DEVELOPMENTAL GENE-EXPRESSION HUMAN GENOME HUMAN-CELLS EFFICIENT ENDONUCLEASE GENERATION KNOCKOUT CAS9 TRANSLOCATIONS
DOI	10.1093/nar/gkt464
英文摘要	Customized TALENs and Cas9/gRNAs have been used for targeted mutagenesis in zebrafish to induce indels into protein-coding genes. However, indels are usually not sufficient to disrupt the function of non-coding genes, gene clusters or regulatory sequences, whereas large genomic deletions or inversions are more desirable for this purpose. By injecting two pairs of TALEN mRNAs or two gRNAs together with Cas9 mRNA targeting distal DNA sites of the same chromosome, we obtained predictable genomic deletions or inversions with sizes ranging from several hundred bases to nearly 1 Mb. We have successfully achieved this type of modifications for 11 chromosomal loci by TALENs and 2 by Cas9/gRNAs with different combinations of gRNA pairs, including clusters of miRNA and protein-coding genes. Seven of eight TALEN-targeted lines transmitted the deletions and one transmitted the inversion through germ line. Our findings indicate that both TALENs and Cas9/gRNAs can be used as an efficient tool to engineer genomes to achieve large deletions or inversions, including fragments covering multiple genes and non-coding sequences. To facilitate the analyses and application of existing ZFN, TALEN and CRISPR/Cas data, we have updated our EENdb database to provide a chromosomal view of all reported engineered endonucleases targeting human and zebrafish genomes.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000323050700006&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; SCI(E); PubMed; 160; ARTICLE; 14; e141; 41
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/342666]
专题	生命科学学院
推荐引用方式 GB/T 7714	Xiao, An,Wang, Zhanxiang,Hu, Yingying,et al. Chromosomal deletions and inversions mediated by TALENs and CRISPR/Cas in zebrafish[J]. 核酸研究,2013.
APA	Xiao, An.,Wang, Zhanxiang.,Hu, Yingying.,Wu, Yingdan.,Luo, Zhou.,...&Zhang, Bo.(2013).Chromosomal deletions and inversions mediated by TALENs and CRISPR/Cas in zebrafish.核酸研究.
MLA	Xiao, An,et al."Chromosomal deletions and inversions mediated by TALENs and CRISPR/Cas in zebrafish".核酸研究 (2013).