Enrichment of the β-catenin-TCF complex at the S and G2 phases ensures cell survival and cell cycle progression

doi:10.1242/jcs.146977

CORC > 北京大学 > 生命科学学院

	Enrichment of the β-catenin-TCF complex at the S and G2 phases ensures cell survival and cell cycle progression
	Ding Yajie ; Su Shang ; Tang Weixin ; Zhang Xiaolei ; Chen Shengyao ; Zhu Guixin ; Liang Juan ; Wei Wensheng ; Guo Ye ; Liu Lei ; Chen Ye-Guang ; Wu Wei
刊名	journal of cell science
	2014
关键词	BiFC,Cell cycle,Cell survival,TCF,β-catenin
DOI	10.1242/jcs.146977
英文摘要	Wnt-β-catenin (β-catenin is also known as CTNNB1 in human) signaling through the β-catenin-TCF complex plays crucial roles in tissue homeostasis. Wnt-stimulated β-catenin-TCF complex accumulation in the nucleus regulates cell survival, proliferation and differentiation through the transcription of target genes. Compared with their levels in G1, activation of the receptor LRP6 and cytosolic β-catenin are both upregulated in G2 cells. However, accumulation of the Wnt pathway negative regulator AXIN2 also occurs in this phase. Therefore, it is unclear whether Wnt signaling is active in G2 phase cells. Here, we established a bimolecular fluorescence complementation (BiFC) biosensor system for the direct visualization of the β-catenin-TCF interaction in living cells. Using the BiFC biosensor and co-immunoprecipitation experiments, we demonstrate that levels of the nucleus-localized β-catenin-TCF complex increase during the S and G2 phases, and declines in the next G1 phase. Accordingly, a subset of Wnt target genes is transcribed by the β-catenin-TCF complex during both the S and G2 phases. By contrast, transient inhibition of this complex disturbs both cell survival and G2/M progression. Our results suggest that in S and G2 phase cells, Wnt-β-catenin signaling is highly active and functions to ensure cell survival and cell cycle progression.? 2014. Published by The Company of Biologists Ltd.; SCI(E); PubMed; 0; ARTICLE; wwu@mail.tsinghua.edu.cn; 22; 4833-45; 127
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/342208]
专题	生命科学学院
推荐引用方式 GB/T 7714	Ding Yajie,Su Shang,Tang Weixin,et al. Enrichment of the β-catenin-TCF complex at the S and G2 phases ensures cell survival and cell cycle progression[J]. journal of cell science,2014.
APA	Ding Yajie.,Su Shang.,Tang Weixin.,Zhang Xiaolei.,Chen Shengyao.,...&Wu Wei.(2014).Enrichment of the β-catenin-TCF complex at the S and G2 phases ensures cell survival and cell cycle progression.journal of cell science.
MLA	Ding Yajie,et al."Enrichment of the β-catenin-TCF complex at the S and G2 phases ensures cell survival and cell cycle progression".journal of cell science (2014).