The Development and Application of a Multiplex STR System for Identifying Subspecies, Individuals and Sex in Tigers

doi:10.1111/1749-4877.12136

CORC > 北京大学 > 生命科学学院

	The Development and Application of a Multiplex STR System for Identifying Subspecies, Individuals and Sex in Tigers
	Zou Zheng-Ting ; Uphyrkina Olga ; Fomenko Pavel ; Luo Shu-Jin
刊名	integrative zoology
	2015
关键词	Amur tiger,STR,genetic,multiplex,sexing
DOI	10.1111/1749-4877.12136
英文摘要	Poaching and trans-boundary trafficking of tigers and body parts are threatening the world's last remaining wild tigers. Development of an efficient molecular genetic assay for tracing origins of confiscated specimens will assist in law enforcement and wildlife forensics for this iconic flagship species. We developed the multiplex genotyping system "tigrisPlex" to simultaneously assess 22 STR (Short Tandem Repeat, or microsatellite) loci and a gender-identifying SRY gene, all amplified in four reactions using as little as 1 ng of template DNA. With DNA samples used for between-run calibration, the system generates STR genotypes that are directly compatible with voucher tiger subspecies genetic profiles, hence making it possible to identify subspecies via bi-parentally inherited markers. We applied "tigrisPlex" to 12 confiscated specimens from Russia and identified six individuals (3 females and 3 males), each represented by duplicated samples and all designated as Amur tigers (Panthera tigris altaica) with high confidence. This STR multiplex system can serve as an effective and versatile approach for genetic profiling of both wild and captive tigers as well as confiscated tiger products, fulfilling various conservation needs for identifying the origins of tiger samples. This article is protected by copyright. All rights reserved.This article is protected by copyright. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000358924600007&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; SCI(E); PubMed; 中国科学引文数据库(CSCD); 2; ARTICLE; luo.shujin@pku.edu.cn
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/341238]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zou Zheng-Ting,Uphyrkina Olga,Fomenko Pavel,et al. The Development and Application of a Multiplex STR System for Identifying Subspecies, Individuals and Sex in Tigers[J]. integrative zoology,2015.
APA	Zou Zheng-Ting,Uphyrkina Olga,Fomenko Pavel,&Luo Shu-Jin.(2015).The Development and Application of a Multiplex STR System for Identifying Subspecies, Individuals and Sex in Tigers.integrative zoology.
MLA	Zou Zheng-Ting,et al."The Development and Application of a Multiplex STR System for Identifying Subspecies, Individuals and Sex in Tigers".integrative zoology (2015).