Activation of DegP chaperone-protease via formation of large cage-like   oligomers upon binding to substrate proteins

doi:10.1073/pnas.0805464105

CORC > 北京大学 > 生命科学学院

	Activation of DegP chaperone-protease via formation of large cage-like oligomers upon binding to substrate proteins
	Jiang, Jiansen ; Zhang, Xuefeng ; Chen, Yong ; Wu, Yi ; Zhou, Z. Hong ; Chang, Zengyi ; Sui, Sen-Fang
刊名	proceedings of the national academy of sciences of the united states of america
	2008
关键词	cryo-electron microscopy protein quality control HtrA PDZ domain oligomerization HEAT-SHOCK-PROTEIN ESCHERICHIA-COLI CRYSTAL-STRUCTURE HTRA PROTEASE STRESS SENSOR HIGH-TEMPERATURES SERINE-PROTEASE QUALITY-CONTROL PDZ DOMAINS GENE
DOI	10.1073/pnas.0805464105
英文摘要	Cells use molecular chaperones and proteases to implement the essential quality control mechanism of proteins. The DegP (HtrA) protein, essential for the survival of Escherichia coli cells at elevated temperatures with homologues found in almost all organisms uniquely has both functions. Here we report a mechanism for DegP to activate both functions via formation of large cage-like 12- and 24-mers after binding to substrate proteins. Cryo-electron microscopic and biochemical studies revealed that both oligomers are consistently assembled by blocks of DegP trimers, via pairwise PDZ1-PDZ2 interactions between neighboring trimers. Such interactions simultaneously eliminate the inhibitory effects of the PDZ2 domain. Additionally, both DegP oligomers were also observed in extracts of E. coli cells, strongly implicating their physiological importance.; Multidisciplinary Sciences; SCI(E); 0; ARTICLE; 33; 11939-11944; 105
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/332870]
专题	生命科学学院
推荐引用方式 GB/T 7714	Jiang, Jiansen,Zhang, Xuefeng,Chen, Yong,et al. Activation of DegP chaperone-protease via formation of large cage-like oligomers upon binding to substrate proteins[J]. proceedings of the national academy of sciences of the united states of america,2008.
APA	Jiang, Jiansen.,Zhang, Xuefeng.,Chen, Yong.,Wu, Yi.,Zhou, Z. Hong.,...&Sui, Sen-Fang.(2008).Activation of DegP chaperone-protease via formation of large cage-like oligomers upon binding to substrate proteins.proceedings of the national academy of sciences of the united states of america.
MLA	Jiang, Jiansen,et al."Activation of DegP chaperone-protease via formation of large cage-like oligomers upon binding to substrate proteins".proceedings of the national academy of sciences of the united states of america (2008).