Phytochrome A Mediates Rapid Red Light-Induced Phosphorylation of   Arabidopsis FAR-RED ELONGATED HYPOCOTYL1 in a Low Fluence Response

doi:10.1105/tpc.108.061259

CORC > 北京大学 > 生命科学学院

	Phytochrome A Mediates Rapid Red Light-Induced Phosphorylation of Arabidopsis FAR-RED ELONGATED HYPOCOTYL1 in a Low Fluence Response
	Shen, Yunping ; Zhou, Zhenzhen ; Feng, Suhua ; Li, Jigang ; Tan-Wilson, Anna ; Qu, Li-Jia ; Wang, Haiyang ; Deng, Xing Wang
刊名	plant cell
	2009
关键词	PROTEIN STABILITY FLUORESCENCE COMPLEMENTATION PHYSIOLOGICAL-RESPONSES TRANSCRIPTION FACTORS OVERLAPPING FUNCTIONS NUCLEAR ACCUMULATION SEEDLING DEVELOPMENT SIGNAL TRANSDUCER A PHOTORECEPTOR COP9 COMPLEX
DOI	10.1105/tpc.108.061259
英文摘要	Phytochrome A (phyA) is the primary photoreceptor for mediating the far-red high irradiance response in Arabidopsis thaliana. FAR-RED ELONGATED HYPOCOTYL1 (FHY1) and its homolog FHY1-LIKE (FHL) define two positive regulators in the phyA signaling pathway. These two proteins have been reported to be essential for light-regulated phyA nuclear accumulation through direct physical interaction with phyA. Here, we report that FHY1 protein is phosphorylated rapidly after exposure to red light. Subsequent exposure to far-red light after the red light pulse reverses FHY1 phosphorylation. Such a phenomenon represents a classical red/far-red reversible low fluence response. The phosphorylation of FHY1 depends on functioning phyA but not on other phytochromes and cryptochromes. Furthermore, we demonstrate that FHY1 and FHL directly interact with phyA by bimolecular fluorescence complementation and that both FHY1 and FHL interact more stably with the Pr form of phyA in Arabidopsis seedlings by coimmunoprecipitation. Finally, in vitro kinase assays confirmed that a recombinant phyA is able to robustly phosphorylate FHY1. Together, our results suggest that phyA may differentially regulate FHY1 and FHL activity through direct physical interaction and red/far-red light reversible phosphorylation to fine-tune their degradation rates and resulting light responses.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000264706500014&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; Plant Sciences; Cell Biology; SCI(E); 33; ARTICLE; 2; 494-506; 21
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/320255]
专题	生命科学学院
推荐引用方式 GB/T 7714	Shen, Yunping,Zhou, Zhenzhen,Feng, Suhua,et al. Phytochrome A Mediates Rapid Red Light-Induced Phosphorylation of Arabidopsis FAR-RED ELONGATED HYPOCOTYL1 in a Low Fluence Response[J]. plant cell,2009.
APA	Shen, Yunping.,Zhou, Zhenzhen.,Feng, Suhua.,Li, Jigang.,Tan-Wilson, Anna.,...&Deng, Xing Wang.(2009).Phytochrome A Mediates Rapid Red Light-Induced Phosphorylation of Arabidopsis FAR-RED ELONGATED HYPOCOTYL1 in a Low Fluence Response.plant cell.
MLA	Shen, Yunping,et al."Phytochrome A Mediates Rapid Red Light-Induced Phosphorylation of Arabidopsis FAR-RED ELONGATED HYPOCOTYL1 in a Low Fluence Response".plant cell (2009).