Assembly and structure of neurofilaments isolated from bovine spinal   cord

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	Assembly and structure of neurofilaments isolated from bovine spinal cord
	Tong, XJ ; Chen, JG ; Liu, J ; Pang, SJ ; Zhai, ZH
刊名	science in china series c life sciences
	1999
关键词	neurofilaments (NFs) STM sidearm structure assembly immunogold END DOMAINS ARCHITECTURE FILAMENTS PROTEIN
英文摘要	Neurofilaments (NFs) are neuron-specific intermediate filaments. The NFs were isolated from bovine spinal cord by differential centrifugation. The NFs were detected with electron microscopy and scanning tunneling microscopy (STM). Under STM, two kinds of sidearm of NFs were revealed: one was short, the other was long. They were arrayed along the 10-nm width core filaments one by one. The intervals between two adjacent long sidearms or two short sidearms were 20-22 nm, while those between two adjacent long and short sidearms were 10-11 nm. It was proposed that the rod domain of NF triplet proteins was 3/4-staggered. The assembly properties of NF triplet proteins were also studied. Immuno-colloidal-gold labeling assay showed that NF-M and NF-H are able to co-assemble into long filaments with NF-L. NF-M and NF-H can also co-constitute into winding filaments.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000082559200002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biology; SCI(E); 1; 3; 233-239; 42
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/320098]
专题	生命科学学院
推荐引用方式 GB/T 7714	Tong, XJ,Chen, JG,Liu, J,et al. Assembly and structure of neurofilaments isolated from bovine spinal cord[J]. science in china series c life sciences,1999.
APA	Tong, XJ,Chen, JG,Liu, J,Pang, SJ,&Zhai, ZH.(1999).Assembly and structure of neurofilaments isolated from bovine spinal cord.science in china series c life sciences.
MLA	Tong, XJ,et al."Assembly and structure of neurofilaments isolated from bovine spinal cord".science in china series c life sciences (1999).