Assembly and structure of neurofilaments isolated from bovine spinal cord | |
Tong, XJ ; Chen, JG ; Liu, J ; Pang, SJ ; Zhai, ZH | |
刊名 | science in china series c life sciences
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1999 | |
关键词 | neurofilaments (NFs) STM sidearm structure assembly immunogold END DOMAINS ARCHITECTURE FILAMENTS PROTEIN |
英文摘要 | Neurofilaments (NFs) are neuron-specific intermediate filaments. The NFs were isolated from bovine spinal cord by differential centrifugation. The NFs were detected with electron microscopy and scanning tunneling microscopy (STM). Under STM, two kinds of sidearm of NFs were revealed: one was short, the other was long. They were arrayed along the 10-nm width core filaments one by one. The intervals between two adjacent long sidearms or two short sidearms were 20-22 nm, while those between two adjacent long and short sidearms were 10-11 nm. It was proposed that the rod domain of NF triplet proteins was 3/4-staggered. The assembly properties of NF triplet proteins were also studied. Immuno-colloidal-gold labeling assay showed that NF-M and NF-H are able to co-assemble into long filaments with NF-L. NF-M and NF-H can also co-constitute into winding filaments.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000082559200002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biology; SCI(E); 1; 3; 233-239; 42 |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.pku.edu.cn/handle/20.500.11897/320098] ![]() |
专题 | 生命科学学院 |
推荐引用方式 GB/T 7714 | Tong, XJ,Chen, JG,Liu, J,et al. Assembly and structure of neurofilaments isolated from bovine spinal cord[J]. science in china series c life sciences,1999. |
APA | Tong, XJ,Chen, JG,Liu, J,Pang, SJ,&Zhai, ZH.(1999).Assembly and structure of neurofilaments isolated from bovine spinal cord.science in china series c life sciences. |
MLA | Tong, XJ,et al."Assembly and structure of neurofilaments isolated from bovine spinal cord".science in china series c life sciences (1999). |
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