Purification and characterization of 66-kDa glycoprotein from human   breast carcinoma

doi:10.1111/j.1349-7006.2007.00539.x

CORC > 北京大学 > 生命科学学院

	Purification and characterization of 66-kDa glycoprotein from human breast carcinoma
	Feng, Renqing ; Liu, Xiang ; Guo, Zhenquan ; Li, Jinfeng
刊名	cancer science
	2007
关键词	TUMOR-ASSOCIATED ANTIGENS CANCER PATIENTS C-ERBB-2 ONCOPROTEIN PROGNOSTIC VALUE CEA TAMOXIFEN DIAGNOSIS MARKERS
DOI	10.1111/j.1349-7006.2007.00539.x
英文摘要	We extracted a 66-kDa glycoprotein (GP-1D8) from breast invasive ductal carcinoma tissues. The monoclonal antibody (mAb) against GP-1D8 was prepared in our laboratory. Western blotting with the purified protein using the mAb demonstrated a single band of 66 kDa. Immunocytochemical and immunohistochemical analysis revealed strong expression of GP-1D8 protein in the cytoplasm of MCF-7 cells and different types of breast carcinoma tissues, but GP-1D8 is absent in normal breast and benign breast tumor tissues. Glycosylation analysis showed GP-1D8 contained methylated salic acid. GP-1D8 was identified using mass-spectrometric techniques and N-terminal sequencing. These data were used to identify the protein through the SWISSPROT protein sequence database and BLAST homology search. These results showed GP-1D8 had some similarity to human albumin precursor. Co-immunoprecipitation assays of lysate from MCF-7 cells and mass spectrometric analysis revealed the interaction of GP-1D8 with beta-tubulin. This is the first time human breast carcinoma tissues and MCF-7 cells have been shown to express a 66-kDa glycoprotein similar to human albumin precursor. These results might be important in the detection of novel potential biomarkers and may provide insight into the molecular mechanisms of tumorigenesis.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000248968500010&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Oncology; SCI(E); PubMed; 2; ARTICLE; 9; 1344-1349; 98
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/320020]
专题	生命科学学院
推荐引用方式 GB/T 7714	Feng, Renqing,Liu, Xiang,Guo, Zhenquan,et al. Purification and characterization of 66-kDa glycoprotein from human breast carcinoma[J]. cancer science,2007.
APA	Feng, Renqing,Liu, Xiang,Guo, Zhenquan,&Li, Jinfeng.(2007).Purification and characterization of 66-kDa glycoprotein from human breast carcinoma.cancer science.
MLA	Feng, Renqing,et al."Purification and characterization of 66-kDa glycoprotein from human breast carcinoma".cancer science (2007).