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Co-suppression in transgenic Petunia hybrida expressing chalcone synthase A (chsA)
Li, Y ; Xi, YW ; Zhang, ZK ; Huang, XQ
刊名science in china series c life sciences
2001
关键词chalcone synthase A co-suppression Petunia hybrida in situ hybridization PETAL DEVELOPMENT GENE-EXPRESSION MESSENGER-RNA PLANTS
DOI10.1007/BF02879361
英文摘要Chalcone synthase A is a key enzyme in the anthocyanin biosynthesis pathway. Expression of chsA gene in transgenic Petunia hybrida resulted in flower color alterations and co-suppression of transgenes and endogenous genes. We fused the a-glucuronidase (uidA) gene to the C-terminal of chsA gene, and transferred the fusion gene into Petunia hybrida via Agrobacterium tumefaciens. GUS histochemical staining analysis showed that co-suppression occurred specifically during the development of flowers and co-suppression required the mutual interaction of endogenous genes and transgenes. RNA in situ hybridization analysis suggested that co-suppression occurred in the entire plant, and RNA degradation occurred in the cytoplasm.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000175029600013&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biology; SCI(E); PubMed; 3; ARTICLE; 6; 661-+; 44
语种英语
内容类型期刊论文
源URL[http://ir.pku.edu.cn/handle/20.500.11897/319996]  
专题生命科学学院
推荐引用方式
GB/T 7714
Li, Y,Xi, YW,Zhang, ZK,et al. Co-suppression in transgenic Petunia hybrida expressing chalcone synthase A (chsA)[J]. science in china series c life sciences,2001.
APA Li, Y,Xi, YW,Zhang, ZK,&Huang, XQ.(2001).Co-suppression in transgenic Petunia hybrida expressing chalcone synthase A (chsA).science in china series c life sciences.
MLA Li, Y,et al."Co-suppression in transgenic Petunia hybrida expressing chalcone synthase A (chsA)".science in china series c life sciences (2001).
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