Co-suppression in transgenic Petunia hybrida expressing chalcone synthase A (chsA) | |
Li, Y ; Xi, YW ; Zhang, ZK ; Huang, XQ | |
刊名 | science in china series c life sciences
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2001 | |
关键词 | chalcone synthase A co-suppression Petunia hybrida in situ hybridization PETAL DEVELOPMENT GENE-EXPRESSION MESSENGER-RNA PLANTS |
DOI | 10.1007/BF02879361 |
英文摘要 | Chalcone synthase A is a key enzyme in the anthocyanin biosynthesis pathway. Expression of chsA gene in transgenic Petunia hybrida resulted in flower color alterations and co-suppression of transgenes and endogenous genes. We fused the a-glucuronidase (uidA) gene to the C-terminal of chsA gene, and transferred the fusion gene into Petunia hybrida via Agrobacterium tumefaciens. GUS histochemical staining analysis showed that co-suppression occurred specifically during the development of flowers and co-suppression required the mutual interaction of endogenous genes and transgenes. RNA in situ hybridization analysis suggested that co-suppression occurred in the entire plant, and RNA degradation occurred in the cytoplasm.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000175029600013&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biology; SCI(E); PubMed; 3; ARTICLE; 6; 661-+; 44 |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.pku.edu.cn/handle/20.500.11897/319996] ![]() |
专题 | 生命科学学院 |
推荐引用方式 GB/T 7714 | Li, Y,Xi, YW,Zhang, ZK,et al. Co-suppression in transgenic Petunia hybrida expressing chalcone synthase A (chsA)[J]. science in china series c life sciences,2001. |
APA | Li, Y,Xi, YW,Zhang, ZK,&Huang, XQ.(2001).Co-suppression in transgenic Petunia hybrida expressing chalcone synthase A (chsA).science in china series c life sciences. |
MLA | Li, Y,et al."Co-suppression in transgenic Petunia hybrida expressing chalcone synthase A (chsA)".science in china series c life sciences (2001). |
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