Pins homolog LGN regulates meiotic spindle organization in mouse oocytes

doi:10.1038/cr.2009.54

CORC > 北京大学 > 生命科学学院

	Pins homolog LGN regulates meiotic spindle organization in mouse oocytes
	Guo, Xinzheng ; Gao, Shaorong
刊名	细胞研究英文版
	2009
关键词	LGN oocyte cortex polarization meiotic spindle organization gamma-tubulin MOTOR PROTEINS CELL-DIVISION POLES DROSOPHILA POLARITY NUMA INTERFERENCE LOCALIZATION CENTROSOMES PROGENITORS
DOI	10.1038/cr.2009.54
英文摘要	Mouse oocytes undergo polarization during meiotic maturation, and this polarization is essential for asymmetric cell divisions that maximize retention of maternal components required for early development. Without conventional centrosomes, the meiotic spindle has less focused poles and is barrel-shaped. The migration of meiotic spindles to the cortex is accompanied by a local reorganization and polarization of the cortex. LGN is a conserved protein involved in cell polarity and regulation of spindle organization. In the present study, we characterized the localization dynamics of LGN during mouse oocyte maturation and analyzed the effects of LGN upregulation and downregulation on meiotic spindle organization. At the germinal vesicle stage, LGN is distributed both cytoplasmically and at the cortex. During maturation, LGN localizes to the meiotic spindle apparatus and cortical LGN becomes less concentrated at the actin cap region. Excessive LGN induces meiotic spindle organization defects by elongating the spindle and enhancing pole focusing, whereas depletion of LGN by RNA interference results in meiotic spindle deformation and chromosome misalignment. Furthermore, the N-terminus of LGN has the ability of full-length LGN to regulate spindle organization, whereas the C-terminus of LGN controls cortical localization and polarization. Our results reveal that LGN is cortically polarized in mouse oocytes and is critical for meiotic spindle organization.; Cell Biology; SCI(E); PubMed; 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 11; ARTICLE; 7; 838-848; 19
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/319992]
专题	生命科学学院
推荐引用方式 GB/T 7714	Guo, Xinzheng,Gao, Shaorong. Pins homolog LGN regulates meiotic spindle organization in mouse oocytes[J]. 细胞研究英文版,2009.
APA	Guo, Xinzheng,&Gao, Shaorong.(2009).Pins homolog LGN regulates meiotic spindle organization in mouse oocytes.细胞研究英文版.
MLA	Guo, Xinzheng,et al."Pins homolog LGN regulates meiotic spindle organization in mouse oocytes".细胞研究英文版 (2009).