Inhibition of epithelial to mesenchymal transition in metastatic breast   carcinoma cells by c-Src suppression

doi:10.1093/abbs/gmq043

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	Inhibition of epithelial to mesenchymal transition in metastatic breast carcinoma cells by c-Src suppression
	Liu, Xiang ; Feng, Renqing
刊名	acta biochimica et biophysica sinica
	2010
关键词	c-Src PP2 siRNA breast carcinoma epithelial to mesenchymal transition FOCAL ADHESION KINASE TRANSCRIPTION FACTORS CANCER-CELLS E-CADHERIN TGF-BETA ACTIVATION EXPRESSION SNAIL TWIST SLUG
DOI	10.1093/abbs/gmq043
英文摘要	The aberrant activation of c-Src regulates multiple functions during tumor progression. This study was conducted to investigate the role of c-Src suppression in epithelial to mesenchymal transition (EMT) process in human breast carcinoma cells. c-Src suppression by PP2 (a Src-family kinase inhibitor) or small interfering RNA (siRNA) was carried out in MCF-7 and MDA-MB-231 cells. Cell migration was analyzed by wound-healing assay. The transcription and protein levels of EMT markers and transcription factors were evaluated by reverse transcription-PCR and Western blot analysis, respectively. The changed cell morphology was photographed by light microscope. c-Src suppression by PP2 or siRNA reversed the mesenchymal-like phenotype in MDA-MB-231 cells. E-cadherin was upregulated in MCF-7 and MDA-MB-231 cells after c-Src suppression, whereas vimentin was downregulated in MDA-MB-231 cells. Slug and SIP1 were downregulated after c-Src suppression in MCF-7 and MDA-MB-231 cells, whereas Twist was unchanged. These results suggest that c-Src suppression by PP2 or siRNA may inhibit EMT through regulation of different transcription factors in breast carcinoma cells that have different metastatic potential.; Biochemistry & Molecular Biology; Biophysics; SCI(E); PubMed; 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 14; ARTICLE; 7; 496-501; 42
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/319974]
专题	生命科学学院
推荐引用方式 GB/T 7714	Liu, Xiang,Feng, Renqing. Inhibition of epithelial to mesenchymal transition in metastatic breast carcinoma cells by c-Src suppression[J]. acta biochimica et biophysica sinica,2010.
APA	Liu, Xiang,&Feng, Renqing.(2010).Inhibition of epithelial to mesenchymal transition in metastatic breast carcinoma cells by c-Src suppression.acta biochimica et biophysica sinica.
MLA	Liu, Xiang,et al."Inhibition of epithelial to mesenchymal transition in metastatic breast carcinoma cells by c-Src suppression".acta biochimica et biophysica sinica (2010).