Pericentrin contains five NESs and an NLS essential for its   nucleocytoplasmic trafficking during the cell cycle

doi:10.1038/cr.2010.89

CORC > 北京大学 > 生命科学学院

	Pericentrin contains five NESs and an NLS essential for its nucleocytoplasmic trafficking during the cell cycle
	Liu, Qinying ; Yu, Jingying ; Zhuo, Xiaolong ; Jiang, Qing ; Zhang, Chuanmao
刊名	细胞研究英文版
	2010
关键词	pericentrin NLS NES importin alpha/beta Crm1 NUCLEAR EXPORT SIGNALS MICROTUBULE ORGANIZATION SPINDLE ORGANIZATION IMPORTIN-ALPHA MITOTIC ENTRY PROTEIN CENTROSOME SEQUENCE LOCALIZATION TRANSPORT
DOI	10.1038/cr.2010.89
英文摘要	Pericentrin, a conserved centrosomal component, provides the structural scaffold to anchor numerous centrosomal proteins, and thus plays an essential role in the organization and function of the centrosome and the mitotic spindle. Although pericentrin was shown to localize in the cytoplasm and reported to be sensitive to leptomycin B (LMB), a specific inhibitor of Crm1, the regions within pericentrin that serve as signals for transporting in and out of the nucleus have not yet been identified. In this study, we identified five novel nuclear export signals (NESs) in pericentrin with diverse export activities. All of the five NESs could bind to Crm1 in a LMB-sensitive way when mediating the nuclear export of pericentrin. We also demonstrated that the region of amino acids 8-42 in pericentrin contains a tripartite nuclear localization signal (NLS) consisting of three clusters of basic amino acids. The NLS of pericentrin binds to importin beta directly or via the adaptor importin a to form the import complex, which could be disrupted by RanQ69L, a dominant-negative Ran GTPase possessing high affinity for importin beta. Furthermore, we found that mutation of the NESs in full-length pericentrin results in both nuclear and cytoplasmic localization, and mutation of the NLS abolishes the nuclear import of pericentrin. On the basis of these results, we suggest that the NESs and NLS of pericentrin are essential for its subcellular localization and nucleocytoplasmic trafficking during the cell cycle.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000280563100011&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Cell Biology; SCI(E); PubMed; 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 7; ARTICLE; 8; 948-962; 20
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/319972]
专题	生命科学学院
推荐引用方式 GB/T 7714	Liu, Qinying,Yu, Jingying,Zhuo, Xiaolong,et al. Pericentrin contains five NESs and an NLS essential for its nucleocytoplasmic trafficking during the cell cycle[J]. 细胞研究英文版,2010.
APA	Liu, Qinying,Yu, Jingying,Zhuo, Xiaolong,Jiang, Qing,&Zhang, Chuanmao.(2010).Pericentrin contains five NESs and an NLS essential for its nucleocytoplasmic trafficking during the cell cycle.细胞研究英文版.
MLA	Liu, Qinying,et al."Pericentrin contains five NESs and an NLS essential for its nucleocytoplasmic trafficking during the cell cycle".细胞研究英文版 (2010).