ARF-like Protein 16 (ARL16) Inhibits RIG-I by Binding with Its   C-terminal Domain in a GTP-dependent Manner

doi:10.1074/jbc.M110.206896

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	ARF-like Protein 16 (ARL16) Inhibits RIG-I by Binding with Its C-terminal Domain in a GTP-dependent Manner
	Yang, Yong-Kang ; Qu, Hong ; Gao, Dong ; Di, Wei ; Chen, Hai-Wei ; Guo, Xin ; Zhai, Zhong-He ; Chen, Dan-Ying
刊名	journal of biological chemistry
	2011
关键词	NEGATIVE FEEDBACK-REGULATION ANTIVIRAL INNATE IMMUNITY E3 UBIQUITIN LIGASE NF-KAPPA-B ADAPTER PROTEIN SWISS-MODEL ENDOPLASMIC-RETICULUM GOLGI-APPARATUS RNA HELICASE SAR PROTEINS
DOI	10.1074/jbc.M110.206896
英文摘要	Retinoic acid-inducible gene I (RIG-I) recognizes RNA virus-derived nucleic acids, which leads to the production of type I interferon (IFN) in most cell types. Tight regulation of RIG-I activity is important to prevent ultra-immune responses. In this study, we identified an ARF-like (ARL) family member, ARL16, as a protein that interacts with RIG-I. Overexpression of ARL16, but not its homologous proteins ARL1 and ARF1, inhibited RIG-I-mediated downstream signaling and antiviral activity. Knockdown of endogenous ARL16 by RNAi potentiated Sendai virus-induced IFN-beta expression and vesicular stomatitis virus replication. ARL16 interacted with the C-terminal domain (CTD) of RIG-I to suppress the association between RIG-I and RNA. ARL16 (T37N) and ARL16 Delta 45-54, which were restricted to the GTP-disassociated form, did not interact with RIG-I and also lost the inhibitory function. Furthermore, we suggest that endogenous ARL16 changes to GTP binding status upon viral infection and binds with the RIG-I CTD to negatively control its signaling activity. These findings suggested a novel innate immune function for an ARL family member, and a GTP-dependent model in which RIG-I is regulated.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000288547000072&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; SCI(E); EI; PubMed; 10; ARTICLE; 12; 10568-10580; 286
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/319967]
专题	生命科学学院
推荐引用方式 GB/T 7714	Yang, Yong-Kang,Qu, Hong,Gao, Dong,et al. ARF-like Protein 16 (ARL16) Inhibits RIG-I by Binding with Its C-terminal Domain in a GTP-dependent Manner[J]. journal of biological chemistry,2011.
APA	Yang, Yong-Kang.,Qu, Hong.,Gao, Dong.,Di, Wei.,Chen, Hai-Wei.,...&Chen, Dan-Ying.(2011).ARF-like Protein 16 (ARL16) Inhibits RIG-I by Binding with Its C-terminal Domain in a GTP-dependent Manner.journal of biological chemistry.
MLA	Yang, Yong-Kang,et al."ARF-like Protein 16 (ARL16) Inhibits RIG-I by Binding with Its C-terminal Domain in a GTP-dependent Manner".journal of biological chemistry (2011).