Imaging superoxide flash and metabolism-coupled mitochondrial   permeability transition in living animals

doi:10.1038/cr.2011.81

CORC > 北京大学 > 生命科学学院

	Imaging superoxide flash and metabolism-coupled mitochondrial permeability transition in living animals
	Fang, Huaqiang ; Chen, Min ; Ding, Yi ; Shang, Wei ; Xu, Jiejia ; Zhang, Xing ; Zhang, Wanrui ; Li, Kaitao ; Xiao, Yao ; Gao, Feng ; Shang, Shujiang ; Li, Jing-Chao ; Tian, Xiao-Li ; Wang, Shi-Qiang ; Zhou, Jingsong ; Weisleder, Noah ; Ma, Jianjie ; Ouyang, Kunfu ; Chen, Ju ; Wang, Xianhua ; Zheng, Ming ; Wang, Wang ; Zhang, Xiuqin ; Cheng, Heping
刊名	细胞研究英文版
	2011
关键词	reactive oxygen species mitochondrial permeability transition energy metabolism in vivo molecular imaging SKELETAL-MUSCLE OXIDATIVE STRESS INSULIN-RESISTANCE HYDROGEN-PEROXIDE IN-VIVO RETICULUM ARRANGEMENT FIBERS PROBES DEATH
DOI	10.1038/cr.2011.81
英文摘要	The mitochondrion is essential for energy metabolism and production of reactive oxygen species (ROS). In intact cells, respiratory mitochondria exhibit spontaneous "superoxide flashes", the quantal ROS-producing events consequential to transient mitochondrial permeability transition (tMPT). Here we perform the first in vivo imaging of mitochondrial superoxide flashes and tMPT activity in living mice expressing the superoxide biosensor mt-cpYFP, and demonstrate their coupling to whole-body glucose metabolism. Robust tMPT/superoxide flash activity occurred in skeletal muscle and sciatic nerve of anesthetized transgenic mice. In skeletal muscle, imaging tMPT/superoxide flashes revealed labyrinthine three-dimensional networks of mitochondria that operate synchronously. The tMPT/superoxide flash activity surged in response to systemic glucose challenge or insulin stimulation, in an apparently frequency-modulated manner and involving also a shift in the gating mode of tMPT. Thus, in vivo imaging of tMPT-dependent mitochondrial ROS signals and the discovery of the metabolism-tMPT-superoxide flash coupling mark important technological and conceptual advances for the study of mitochondrial function and ROS signaling in health and disease.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000294492500005&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Cell Biology; SCI(E); PubMed; 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 47; ARTICLE; 9; 1295-1304; 21
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/319963]
专题	生命科学学院
推荐引用方式 GB/T 7714	Fang, Huaqiang,Chen, Min,Ding, Yi,et al. Imaging superoxide flash and metabolism-coupled mitochondrial permeability transition in living animals[J]. 细胞研究英文版,2011.
APA	Fang, Huaqiang.,Chen, Min.,Ding, Yi.,Shang, Wei.,Xu, Jiejia.,...&Cheng, Heping.(2011).Imaging superoxide flash and metabolism-coupled mitochondrial permeability transition in living animals.细胞研究英文版.
MLA	Fang, Huaqiang,et al."Imaging superoxide flash and metabolism-coupled mitochondrial permeability transition in living animals".细胞研究英文版 (2011).