ngs (Notochord Granular Surface) Gene Encodes a Novel Type of   Intermediate Filament Family Protein Essential for Notochord Maintenance   in Zebrafish

doi:10.1074/jbc.M112.379172

CORC > 北京大学 > 生命科学学院

	ngs (Notochord Granular Surface) Gene Encodes a Novel Type of Intermediate Filament Family Protein Essential for Notochord Maintenance in Zebrafish
	Tong, Xiangjun ; Xia, Zhidan ; Zu, Yao ; Telfer, Helena ; Hu, Jing ; Yu, Jingyi ; Liu, Huan ; Zhang, Quan ; Sodmergen ; Lin, Shuo ; Zhang, Bo
刊名	journal of biological chemistry
	2013
关键词	EPIDERMOLYSIS-BULLOSA SIMPLEX ALEXANDER-DISEASE MUTATIONS EMBRYOS CELLS MICE GFAP
DOI	10.1074/jbc.M112.379172
英文摘要	The notochord is an important organ involved in embryonic patterning and locomotion. In zebrafish, the mature notochord consists of a single stack of fully differentiated, large vacuolated cells called chordocytes, surrounded by a single layer of less differentiated notochordal epithelial cells called chordoblasts. Through genetic analysis of zebrafish lines carrying pseudo-typed retroviral insertions, a mutant exhibiting a defective notochord with a granular appearance was isolated, and the corresponding gene was identified as ngs (notochord granular surface), which was specifically expressed in the notochord. In the mutants, the notochord started to degenerate from 32 hours post-fertilization, and the chordocytes were then gradually replaced by smaller cells derived from chordoblasts. The granular notochord phenotype was alleviated by anesthetizing the mutant embryos with tricaine to prevent muscle contraction and locomotion. Phylogenetic analysis showed that ngs encodes a new type of intermediate filament (IF) family protein, which we named chordostatin based on its function. Under the transmission electron microcopy, bundles of 10-nm-thick IF-like filaments were enriched in the chordocytes of wild-type zebrafish embryos, whereas the chordocytes in ngs mutants lacked IF-like structures. Furthermore, chordostatin-enhanced GFP (EGFP) fusion protein assembled into a filamentous network specifically in chordocytes. Taken together, our work demonstrates that ngs encodes a novel type of IF protein and functions to maintain notochord integrity for larval development and locomotion. Our work sheds light on the mechanisms of notochord structural maintenance, as well as the evolution and biological function of IF family proteins.; Biochemistry & Molecular Biology; SCI(E); EI; PubMed; 0; ARTICLE; 4; 2711-2720; 288
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/319938]
专题	生命科学学院
推荐引用方式 GB/T 7714	Tong, Xiangjun,Xia, Zhidan,Zu, Yao,et al. ngs (Notochord Granular Surface) Gene Encodes a Novel Type of Intermediate Filament Family Protein Essential for Notochord Maintenance in Zebrafish[J]. journal of biological chemistry,2013.
APA	Tong, Xiangjun.,Xia, Zhidan.,Zu, Yao.,Telfer, Helena.,Hu, Jing.,...&Zhang, Bo.(2013).ngs (Notochord Granular Surface) Gene Encodes a Novel Type of Intermediate Filament Family Protein Essential for Notochord Maintenance in Zebrafish.journal of biological chemistry.
MLA	Tong, Xiangjun,et al."ngs (Notochord Granular Surface) Gene Encodes a Novel Type of Intermediate Filament Family Protein Essential for Notochord Maintenance in Zebrafish".journal of biological chemistry (2013).