Fast Super-Resolution Imaging with Ultra-High Labeling Density Achieved   by Joint Tagging Super-Resolution Optical Fluctuation Imaging

doi:10.1038/srep08359

CORC > 北京大学 > 生命科学学院

	Fast Super-Resolution Imaging with Ultra-High Labeling Density Achieved by Joint Tagging Super-Resolution Optical Fluctuation Imaging
	Zeng, Zhiping ; Chen, Xuanze ; Wang, Hening ; Huang, Ning ; Shan, Chunyan ; Zhang, Hao ; Teng, Junlin ; Xi, Peng
刊名	scientific reports
	2015
关键词	STIMULATED-EMISSION-DEPLETION LOCALIZATION MICROSCOPY QUANTUM DOTS FLUORESCENCE MICROSCOPY STRUCTURED-ILLUMINATION RESOLUTION NANOSCOPY STORM SOFI RECONSTRUCTION
DOI	10.1038/srep08359
英文摘要	Previous stochastic localization-based super-resolution techniques are largely limited by the labeling density and the fidelity to the morphology of specimen. We report on an optical super-resolution imaging scheme implementing joint tagging using multiple fluorescent blinking dyes associated with super-resolution optical fluctuation imaging (JT-SOFI), achieving ultra-high labeling density super-resolution imaging. To demonstrate the feasibility of JT-SOFI, quantum dots with different emission spectra were jointly labeled to the tubulin in COS7 cells, creating ultra-high density labeling. After analyzing and combining the fluorescence intermittency images emanating from spectrally resolved quantum dots, the microtubule networks are capable of being investigated with high fidelity and remarkably enhanced contrast at sub-diffraction resolution. The spectral separation also significantly decreased the frame number required for SOFI, enabling fast super-resolution microscopy through simultaneous data acquisition. As the joint-tagging scheme can decrease the labeling density in each spectral channel, thereby bring it closer to single-molecule state, we can faithfully reconstruct the continuous microtubule structure with high resolution through collection of only 100 frames per channel. The improved continuity of the microtubule structure is quantitatively validated with image skeletonization, thus demonstrating the advantage of JT-SOFI over other localization-based super-resolution methods.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000349172800003&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Multidisciplinary Sciences; SCI(E); PubMed; 8; ARTICLE; xipeng@pku.edu.cn; 8359; 5
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/315610]
专题	生命科学学院工学院
推荐引用方式 GB/T 7714	Zeng, Zhiping,Chen, Xuanze,Wang, Hening,et al. Fast Super-Resolution Imaging with Ultra-High Labeling Density Achieved by Joint Tagging Super-Resolution Optical Fluctuation Imaging[J]. scientific reports,2015.
APA	Zeng, Zhiping.,Chen, Xuanze.,Wang, Hening.,Huang, Ning.,Shan, Chunyan.,...&Xi, Peng.(2015).Fast Super-Resolution Imaging with Ultra-High Labeling Density Achieved by Joint Tagging Super-Resolution Optical Fluctuation Imaging.scientific reports.
MLA	Zeng, Zhiping,et al."Fast Super-Resolution Imaging with Ultra-High Labeling Density Achieved by Joint Tagging Super-Resolution Optical Fluctuation Imaging".scientific reports (2015).