Establishment of mouse model with humanized Philadelphia chromosome-positive acute lymphoblastic leukemia

CORC > 北京大学 > 生命科学学院

	Establishment of mouse model with humanized Philadelphia chromosome-positive acute lymphoblastic leukemia
	Kong Yuan ; Wang Ya-Zhe ; Hu Yue ; Huang Xiao-Jun
刊名	zhongguo shi yan xue ye xue za zhi zhongguo bing li sheng li xue hui
	2014
英文摘要	The purpose of this study was to establish a novel xenotransplant mouse model with human Philadelphia chromosome-positive acute lymphoblastic leukemia (Ph(+)ALL). The bone marrow mononuclear cells (BMMNC) were separated from newly diagnosed Ph(+)ALL patients, and injected into 2.1 Gy of (60)Co irradiated and anti-CD122-conditioned NOD/SCID mice through intra femoral injection. Human hematopoietic chimerism in bone marrow and spleen of the recipients was detected by flow cytometry. Morphological analysis of murine marrow cells were performed using May-Giemsa staining. BCR/ABL1 level was detected by RQ-PCR and FISH assays. Furthermore, leukemia infiltration in the organs was evaluated by hematoxylin-eosin staining, immunohistochemical staining with anti-human CD19 and anti-human CD34 antibodies. The results indicated that the unsorted BMMNC from Ph(+)ALL patients were able to repopulate human Ph(+)ALL in vivo. The percentages of human CD45(+)CD19(+) cells in bone marrow, and spleen of the recipient mice were 87.2% ± 10.1% and 79.9% ± 9.2%, respectively. Furthermore, the engrafted cells possessed same morphology, phenotypic and cytogenetic characteristics as cells from the original Ph(+)ALL patients. Compatible with the clinical features, transplanted Ph(+)ALL cells infiltrated into the brain, liver, and kidney of the recipients. It is concluded that the human-mouse xenotransplant established model using intra femoral injection of an anti-CD122-conditioned NOD/SCID repopulation may provide a promising system to study the biology of human Ph(+)ALL in vivo.; PubMed; 0; 1; 73-7; 22
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/307007]
专题	生命科学学院
推荐引用方式 GB/T 7714	Kong Yuan,Wang Ya-Zhe,Hu Yue,et al. Establishment of mouse model with humanized Philadelphia chromosome-positive acute lymphoblastic leukemia[J]. zhongguo shi yan xue ye xue za zhi zhongguo bing li sheng li xue hui,2014.
APA	Kong Yuan,Wang Ya-Zhe,Hu Yue,&Huang Xiao-Jun.(2014).Establishment of mouse model with humanized Philadelphia chromosome-positive acute lymphoblastic leukemia.zhongguo shi yan xue ye xue za zhi zhongguo bing li sheng li xue hui.
MLA	Kong Yuan,et al."Establishment of mouse model with humanized Philadelphia chromosome-positive acute lymphoblastic leukemia".zhongguo shi yan xue ye xue za zhi zhongguo bing li sheng li xue hui (2014).