Construction and validation of a Sinorhizobium meliloti whole genome DNA microarray: Genome-wide profiling of osmoadaptive gene expression

doi:10.1016/j.jbiotec.2003.08.005

CORC > 北京大学 > 生命科学学院

	Construction and validation of a Sinorhizobium meliloti whole genome DNA microarray: Genome-wide profiling of osmoadaptive gene expression
	R??berg, Silvia ; Tian, Zhe-Xian ; Krol, Elizaveta ; Linke, Burkhard ; Meyer, Folker ; Wang, Yiping ; P??hler, Alfred ; Weidner, Stefan ; Becker, Anke
刊名	journal of biotechnology
	2003
DOI	10.1016/j.jbiotec.2003.08.005
英文摘要	Based on the complete Sinorhizobium meliloti genome sequence we established DNA microarrays as a comprehensive tool for systematic genome-wide gene expression analysis in S. meliloti 1021. For these PCR fragment-based microarrays, called Sm6kPCR, a collection of probes for the 6207 predicted protein-coding genes consisting of 6046 gene-specific PCR fragments and 161 70mer oligonucleotides was arrayed in high density on glass slides. To obtain these PCR fragments primer pairs were designed to amplify internal gene-specific DNA fragments of 80-350bp. Additionally, these primers were characterized by a 5?? extension that allowed for reamplification using standard primers after the first amplification employing the specific primers. In order to ascertain the quality of the Sm6kPCR microarrays and to validate gene expression studies in S. meliloti parallel hybridizations based on RNA samples obtained from cells cultured under identical conditions were performed. In addition, gene expression in S. meliloti in response to an osmotic upshift imposed by the addition of 0.38M NaCl was monitored. 137 genes were identified showing significant changes in gene expression resulting from the osmotic upshift. From these genes 52 were induced and 85 genes were repressed. Among the genes displaying different RNA levels some functional groups could be identified that are particularly remarkable. Repression was observed for 8 genes related to motility and chemotaxis, 7 genes encoding amino acid biosynthesis enzymes and 15 genes involved in iron uptake whereas 14 genes involved in transport of small molecules and 4 genes related to polysaccharide biosynthesis were induced. ? 2003 Elsevier B.V. All rights reserved.; EI; 0; 2-3; 255-268; 106
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/262650]
专题	生命科学学院
推荐引用方式 GB/T 7714	R??berg, Silvia,Tian, Zhe-Xian,Krol, Elizaveta,et al. Construction and validation of a Sinorhizobium meliloti whole genome DNA microarray: Genome-wide profiling of osmoadaptive gene expression[J]. journal of biotechnology,2003.
APA	R??berg, Silvia.,Tian, Zhe-Xian.,Krol, Elizaveta.,Linke, Burkhard.,Meyer, Folker.,...&Becker, Anke.(2003).Construction and validation of a Sinorhizobium meliloti whole genome DNA microarray: Genome-wide profiling of osmoadaptive gene expression.journal of biotechnology.
MLA	R??berg, Silvia,et al."Construction and validation of a Sinorhizobium meliloti whole genome DNA microarray: Genome-wide profiling of osmoadaptive gene expression".journal of biotechnology (2003).