Cloning and expression analysis of the heavy-metal responsive gene PvSR2   from bean

doi:10.1016/S0168-9452(01)00470-8

CORC > 北京大学 > 生命科学学院

	Cloning and expression analysis of the heavy-metal responsive gene PvSR2 from bean
	Zhang, YX ; Chai, TY ; Dong, J ; Zhao, WM ; An, CC ; Chen, ZL ; Burkard, G
刊名	plant science
	2001
关键词	heavy metal gene expression Phaseolus vulgaris Ll ALUMINUM-INDUCED GENES OXIDATIVE STRESS CADMIUM STRESS ARABIDOPSIS IDENTIFICATION TOLERANCE PLANTS
DOI	10.1016/S0168-9452(01)00470-8
英文摘要	A heavy metal responsive gene PvSR2 (Phaseolus vulgaris stress related) which encodes a new heavy metal stress related protein was isolated from a mercuric-chloride-treated bean (Phaseolus vulgaris L.) cDNA library by differential screening using cDNAs derived from treated and untreated plants. The PvSR2 cDNA contains 690 bp, which is about 200 bp shorter than the full length mRNA. Genomic DNA clone sequence analysis showed that PvSR2 gene contains no introns in the coding region. The deduced protein with a calculated molecular weight of 18.5 kDa. The cDNA and protein sequence of PvSR2 has no homology with any known protein. A single copy of PvSR2 was found in bean genomic DNA by Southern blot analysis. Northern blot analysis revealed that PvSR2 mRNA is hardly detectable in unstressed bean seedlings. The gene expression was strongly stimulated by heavy metals, such as mercury, cadmium, arsenic and copper, whereas it was not responsive to any other forms of environment stresses, such as ultraviolet radiation, elevated temperature and virus infection. PvSR2 gene was cloned into a prokaryotic expression vector pBV221 with a PRPL promoter upstream. The heavy metal resistance assay of E coli DH5 alpha strain showed that the transformant DH5a of pBV221-PvSR2 recombinant plasmid displayed obviously resistance to CdCl2 compared with the transformant of pBV221 plasmid (negative control) and the DH5 alpha (control). These suggested that the PvSR2 is a specific heavy metal responsive gene and might play an important role in resistance to the damage caused by heavy metals. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.; Biochemistry & Molecular Biology; Plant Sciences; SCI(E); EI; 12; ARTICLE; 4; 783-790; 161
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/258101]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zhang, YX,Chai, TY,Dong, J,et al. Cloning and expression analysis of the heavy-metal responsive gene PvSR2 from bean[J]. plant science,2001.
APA	Zhang, YX.,Chai, TY.,Dong, J.,Zhao, WM.,An, CC.,...&Burkard, G.(2001).Cloning and expression analysis of the heavy-metal responsive gene PvSR2 from bean.plant science.
MLA	Zhang, YX,et al."Cloning and expression analysis of the heavy-metal responsive gene PvSR2 from bean".plant science (2001).