Identification of inhibitor of apoptosis specific DNase in Xenopus egg   extract

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	Identification of inhibitor of apoptosis specific DNase in Xenopus egg extract
	Lu, ZG ; Yang, W ; Cao, Q ; Tao, W ; Hu, JC ; Zhai, ZH
刊名	科学通报英文版
	2001
关键词	Xenopus laevis apoptosis IXAD XAD DNA Ladder cytochrome c cell-free system CASPASE-ACTIVATED DNASE CELL-FREE APOPTOSIS FRAGMENTATION FACTOR REQUIREMENT DEGRADATION PROTEIN
英文摘要	When added with cytochrome c, Xenopus laevis egg extract XS-150 can induce exogenous nuclei undergoing apoptosis. Apoptosis specific DNase XAD was activated during this process, and cut chromatin between nucleosome, leading to DNA Ladder in electrophoresis. Our results showed that an inhibitor of XAD, IXAD, exists abundantly in normal egg extract, its molecular weight is about 40 ku. Normally, IXAD exists either in the form of dimmer or in complex with NAD. It was degraded during apoptosis, releasing active XAD. The results of Western assay and cross-inhibition showed that MAD was likely homologous to DFF45 in structure and function. At the same time, these results also indicated that the pathway in apoptosis was conserved in evolution.; Multidisciplinary Sciences; SCI(E); 2; ARTICLE; 17; 1449-1453; 46
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/258092]
专题	生命科学学院
推荐引用方式 GB/T 7714	Lu, ZG,Yang, W,Cao, Q,et al. Identification of inhibitor of apoptosis specific DNase in Xenopus egg extract[J]. 科学通报英文版,2001.
APA	Lu, ZG,Yang, W,Cao, Q,Tao, W,Hu, JC,&Zhai, ZH.(2001).Identification of inhibitor of apoptosis specific DNase in Xenopus egg extract.科学通报英文版.
MLA	Lu, ZG,et al."Identification of inhibitor of apoptosis specific DNase in Xenopus egg extract".科学通报英文版 (2001).