N-terminal of L protein of vesicular stomatitis virus contains a new   signal sequence

doi:10.1360/02wc0490

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	N-terminal of L protein of vesicular stomatitis virus contains a new signal sequence
	Nie, YC ; Ke, YY ; Wang, Z ; Yu, X ; Deng, HK ; Ding, MX
刊名	科学通报英文版
	2003
关键词	vesicular stomatitis virus L protein replicase localization transfection REPLICATION COMPLEXES ENDOPLASMIC-RETICULUM MATRIX PROTEIN MEMBRANES LOCALIZATION PHOSPHOPROTEIN TRANSCRIPTION ENVELOPE
DOI	10.1360/02wc0490
英文摘要	The L protein (241 kD) of vesicular stomatitis virus (VSV) is the most important subunit of the replication complex. The existence of specific localization signal in the L protein was investigated by making recombinant constructs expressing truncated mutants of the L protein fused to green fluorescent protein (GFP) in transient transfection assays. The chimeric genes encoding varied N-terminal of L and GFP gene were put under the control of T7 promoter or CMV promoter. The fusion proteins were transiently expressed in BHK-21, COS-7, CHO or Hep G2 cells. When more than 120 residues were deleted or only 96 residues were kept on the N-terminal, the fusion proteins were shown to be distributed throughout the cells, cytoplasm and nucleus under the confocal microscope. However, other chimeric proteins with 120 or more amino acids were dotted and distributed in the perinuclear regions. And the fusion protein with 96-120 as has the similar distribution. A thirteen-residue peptide QGYSFLHEVDKEA (108-120) was identified as localization signal, whose function would be absolutely distributed with the deficiency of D or V Our results show that there is an independent localizing signal in N-terminal domain of L protein of VSV and this functional signal is conserved in different cell lines.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000184463200016&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Multidisciplinary Sciences; SCI(E); 0; ARTICLE; 13; 1352-1357; 48
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/256480]
专题	生命科学学院
推荐引用方式 GB/T 7714	Nie, YC,Ke, YY,Wang, Z,et al. N-terminal of L protein of vesicular stomatitis virus contains a new signal sequence[J]. 科学通报英文版,2003.
APA	Nie, YC,Ke, YY,Wang, Z,Yu, X,Deng, HK,&Ding, MX.(2003).N-terminal of L protein of vesicular stomatitis virus contains a new signal sequence.科学通报英文版.
MLA	Nie, YC,et al."N-terminal of L protein of vesicular stomatitis virus contains a new signal sequence".科学通报英文版 (2003).