巴西橡胶树初生乳管黄色体微纤维蛋白质与67kD蛋白质的关系(英文)

doi:10.3321/j.issn:1672-9072.2003.02.001

CORC > 北京大学 > 生命科学学院

	巴西橡胶树初生乳管黄色体微纤维蛋白质与67kD蛋白质的关系(英文); Fluctuation of microfibrillar protein level in lutoids of primary laticifers in relation to the 67 kD storage protein in Hevea brasiliensis
	田维敏 ; 韩亚琴 ; 吴继林 ; 胡正海
刊名	植物学报
	2003
关键词	微纤维蛋白质 67kD蛋白质苗生长乳管发育巴西橡胶树 microfibrillar protein 67 kD protein shoot growth laticifer development Hevea brasiliensis
DOI	10.3321/j.issn:1672-9072.2003.02.001
英文摘要	在古铜期的巴西橡胶 (HeveabrasiliensisMull.Arg .)幼茎初生乳管黄色体中存在丰富的微纤维蛋白质。在电子显微镜下 ,微纤维蛋白质呈两种不同的形态 ,分别存在于不同的黄色体中。SDS_PAGE分析表明 ,经等电点纯化的微纤维蛋白质的主要成分是 5 9.5kD和 6 3.5kD蛋白质。使用 6 7kD蛋白质的抗血清的免疫印迹表明 ,5 9.5kD和 6 3.5kD蛋白质与积累在贮藏蛋白质细胞中的 6 7kD蛋白质具有一定程度的免疫相关性 ,且在苗生长发育过程中互为消长。 5 9.5kD和 6 3.5kD蛋白质在古铜期的幼茎中最丰富 ,当新梢茎停止伸长及叶片刚成熟时 ,其含量...; Abundant microfibrillar protein inclusions were present in the lutoids of the primary laticifers in Hevea brasiliensis Mull. Arg. Two forms of the inclusions could be distinguished under the electron microscope, each in separate lutoids. As revealed by SDS-PAGE, the 59.5 kD and 63.5 kD proteins were the major components of the microfibrillar protein purified by isoeleettic point precipitation. Western-blotting analysis indicated that they were immunorelated with the 67 kD protein accumulated in the protein-storing cells. The 59.5 kD and 63.5 kD proteins were abundant in the uppermost part, the stem of new shoot and sustained their abundance during the growth and development of new shoot while their contents decreased remarkably in the lower parts of the trunk, accompanying by the accumulation of 3-5 kinds of proteins with low molecular weights. This fluctuating pattern suggested that the degradation of the 59.5 kD and 63.5 kD proteins had nothing to do with the new shoot growth and may be closely related to the primary laticifer differentiation. The 67 kD protein could not be detected in the young stem of new shoot when its leaves were broze-colored while the protein started to be accumulated in the stem, when the leaves of new shoot had matured, behaving like a typical vegetative storage protein.; SCI(E); 2; 02; 127-130; 45
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/256090]
专题	生命科学学院
推荐引用方式 GB/T 7714	田维敏,韩亚琴,吴继林,等. 巴西橡胶树初生乳管黄色体微纤维蛋白质与67kD蛋白质的关系(英文), Fluctuation of microfibrillar protein level in lutoids of primary laticifers in relation to the 67 kD storage protein in Hevea brasiliensis[J]. 植物学报,2003.
APA	田维敏,韩亚琴,吴继林,&胡正海.(2003).巴西橡胶树初生乳管黄色体微纤维蛋白质与67kD蛋白质的关系(英文).植物学报.
MLA	田维敏,et al."巴西橡胶树初生乳管黄色体微纤维蛋白质与67kD蛋白质的关系(英文)".植物学报 (2003).