Proteomic comparison between human young and old brains by   two-dimensional gel electrophoresis and identification of proteins

doi:10.1016/S0736-5748(03)00037-6

CORC > 北京大学 > 生命科学学院

	Proteomic comparison between human young and old brains by two-dimensional gel electrophoresis and identification of proteins
	Chen, W ; Ji, JG ; Xu, XM ; He, SZ ; Ru, BG
刊名	国际发育神经科学
	2003
关键词	brain aging proteomics two-dimensional gel electrophoresis mass spectrometry MASS SPECTROMETRY DEFICIENT MICE EXPRESSION
DOI	10.1016/S0736-5748(03)00037-6
英文摘要	To investigate molecular mechanisms of human brain aging, brain proteins were isolated from postmortem human young and old brains and profiled by two-dimensional gel electrophoresis (2-DE). With the help of special software, five down-regulated protein spots in two-dimensional gel electrophoresis gels of old brains were found compared with young brains, four of which was identified as a protein similar to peroxiredoxin 2 (accession-numbered as gi 11363 1440), two of stathmin (phosphoprotein p19) and apolipoprotein A-I precursor (apo-AI) by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Eight common proteins, whose expressions were not altered between young and old brains, were also identified. The possible relevance of changes was analyzed. This study shows that the contribution of proteomics could be valuable in experimental gerontology field. (C) 2003 ISDN. Published by Elsevier Science Ltd. All rights reserved.; Developmental Biology; Neurosciences; SCI(E); 44; 4; 209-216; 21
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/255882]
专题	生命科学学院
推荐引用方式 GB/T 7714	Chen, W,Ji, JG,Xu, XM,et al. Proteomic comparison between human young and old brains by two-dimensional gel electrophoresis and identification of proteins[J]. 国际发育神经科学,2003.
APA	Chen, W,Ji, JG,Xu, XM,He, SZ,&Ru, BG.(2003).Proteomic comparison between human young and old brains by two-dimensional gel electrophoresis and identification of proteins.国际发育神经科学.
MLA	Chen, W,et al."Proteomic comparison between human young and old brains by two-dimensional gel electrophoresis and identification of proteins".国际发育神经科学 (2003).