Cloning and expressional analyses of a cinnamoyl CoA reductase cDNA from   rice seedlings

doi:10.1360/03wc0264

CORC > 北京大学 > 生命科学学院

	Cloning and expressional analyses of a cinnamoyl CoA reductase cDNA from rice seedlings
	Bai, Y ; Gong, W ; Liu, TY ; Zhu, YX
刊名	科学通报英文版
	2003
关键词	lignin cinnamoyl CoA reductase vascular bundles Oryza sativa L. ALCOHOL-DEHYDROGENASE ARABIDOPSIS-THALIANA DOWN-REGULATION LIGNIN CCR PURIFICATION BIOSYNTHESIS PHENOTYPE PLANTS GENES
DOI	10.1360/03wc0264
英文摘要	Cinnamoyl CoA reductase (CCR: EC 1.2.1.44), the entry-point enzyme of the lignin specific biosynthetic pathway, catalyzes the conversion of cinnamoyl CoA esters to their corresponding cinnamaldehydes. Multiple sequence alignment showed that the deduced polypeptide shared 70% similarity and 30% sequence identity at the amino acid level with defined CCR genes from other plant species and they all contain the common signature sequences thought to be the catalytic site as well as the putative NADP binding domain. Using a conserved OsCCR cDNA fragment as the probe for library screening, we isolated the genomic DNA that covered the whole coding region of OsCCR with total length of 3045 bp including 4 introns and 5 exons. The open reading frame for our OsCCR gene contains 337 amino acids. Northern blot indicated that OsCCR was expressed in different organs with the highest level found in stems. In situ hybridization results showed that OsCCR mRNA was localized mainly along the vascular bundles in stems and leaves, and also in lateral roots that was differentiating from the tillering node. We conclude that the vascular-localized expression of OsCCR gene may suggest its possible involvement in lignin biosynthesis. Cloning and characterization of OsCCR will help to clarify how lignifications in plants are regulated and will provide a physical basis for creating genetically engineered rice plants with optimal lignin contents.; Multidisciplinary Sciences; SCI(E); 3; ARTICLE; 20; 2221-2225; 48
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/255657]
专题	生命科学学院
推荐引用方式 GB/T 7714	Bai, Y,Gong, W,Liu, TY,et al. Cloning and expressional analyses of a cinnamoyl CoA reductase cDNA from rice seedlings[J]. 科学通报英文版,2003.
APA	Bai, Y,Gong, W,Liu, TY,&Zhu, YX.(2003).Cloning and expressional analyses of a cinnamoyl CoA reductase cDNA from rice seedlings.科学通报英文版.
MLA	Bai, Y,et al."Cloning and expressional analyses of a cinnamoyl CoA reductase cDNA from rice seedlings".科学通报英文版 (2003).