Isolation and characterization of a dehydrin-like gene from   drought-tolerant Boea crassifolia

doi:10.1016/j.plantsci.2003.12.025

CORC > 北京大学 > 生命科学学院

	Isolation and characterization of a dehydrin-like gene from drought-tolerant Boea crassifolia
	Shen, Y ; Tang, MJ ; Hu, YL ; Lin, ZP
刊名	plant science
	2004
关键词	Boea crassifolia mRNA differential display dehydrins abiotic stress wounding stress CIS-ACTING ELEMENT ABSCISIC-ACID METHYL JASMONATE WATER-DEFICIT SIGNAL-TRANSDUCTION MULTIGENE FAMILY EXPRESSION ARABIDOPSIS STRESS DEFENSE
DOI	10.1016/j.plantsci.2003.12.025
英文摘要	A novel dehydrin-like gene, designated BcDh2, was isolated from Boea crassifolia (Hemsley) by using mRNA differential display and rapid amplification of cDNA ends (RACE). The full length of BcDh2 cDNA consists of 700 nucleotides and has an open reading frame of 399 bp. The deduced amino acid sequence of BcDh2 has 133 amino acid residues which forms a 15 kDa polypeptide with a calculated pl of 7.95. The putative polypeptide composed of one consensus Y-segment, a stretch of serine residues and two Lysine-rich repeats which presented a typical YnSK2 structure of dehydrins. The transcripts of BcDh2 highly accumulated when the plants were treated with drought, salinity, exogenous abscisic acid (ABA) and moderated heat shock, while accumulated slightly in response to low temperature stress. BcDh2 also accumulated slightly in response to wounding signals such as MeJA and low concentration of salicylic acid. For further investigation, the promoter region of BcDh2 gene isolated was fused to the beta-glucuronidase reporter gene (GUS) and transferred into tobacco (W38). The assays of GUS activity in transgenic plants treated by drought, cold, heat, exogenous ABA and MeJA were in accord with Northern analysis of BcDh2 mRNA in B. crassifolia. (C) 2004 Elsevier Ireland Ltd. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000220838600007&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; Plant Sciences; SCI(E); EI; 16; ARTICLE; 5; 1167-1175; 166
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/255077]
专题	生命科学学院
推荐引用方式 GB/T 7714	Shen, Y,Tang, MJ,Hu, YL,et al. Isolation and characterization of a dehydrin-like gene from drought-tolerant Boea crassifolia[J]. plant science,2004.
APA	Shen, Y,Tang, MJ,Hu, YL,&Lin, ZP.(2004).Isolation and characterization of a dehydrin-like gene from drought-tolerant Boea crassifolia.plant science.
MLA	Shen, Y,et al."Isolation and characterization of a dehydrin-like gene from drought-tolerant Boea crassifolia".plant science (2004).