Cloning and characterization of a drought-inducible MYB gene from Boea   crassifolia

doi:10.1016/j.plantsci.2004.09.013

CORC > 北京大学 > 生命科学学院

	Cloning and characterization of a drought-inducible MYB gene from Boea crassifolia
	Chen, BJ ; Wang, Y ; Hu, YL ; Wu, Q ; Lin, ZP
刊名	plant science
	2005
关键词	Boea crassifolia MYB transcription factor drought-inducible abiotic stress RT-PCR TRANSCRIPTION FACTORS ARABIDOPSIS-THALIANA LOW-TEMPERATURE ABSCISIC-ACID MOLECULAR RESPONSES WATER-DEFICIT EXPRESSION STRESS PLANTS PROMOTER
DOI	10.1016/j.plantsci.2004.09.013
英文摘要	A drought-inducible MYB gene, designated BcMYBI (GenBank accession no. AY278440), was isolated from Boea crassifolia (Hemsley) by RT-PCR and reverse northern blot screen. The full-length cDNA of BcMYBI consists of 1167 nucleotides and has an open reading frame of 1023 bp, encoding a typical R2R3-MYB transcription factor belonging to the subgroup I I of the R2R3-MYB protein family. RNA gel blot analysis showed that BcMYBI was strongly induced by drought stress, and also responded to PEG, high salinity and low temperature to some extent. However, BcMYBI transcripts did not or just slightly accumulated upon treatments with exogenous abscisic acid (ABA), methyl jasmonate (MeJa) and salicylic acid (SA). These results indicate that BcMYBI might be involved in the regulation of gene expression in response to dehydration stress through an ABA-independent pathway, whereas it seems not to be a regulatory component in wounding signaling. In addition, the 1007 bp upstream regulatory region of BcMYBI was obtained by PCR-based chromosome walking, and sequence analysis of the region predicted some putative cis-elements related to abiotic stress response. Chimeric beta-glucuronidase gene (GUS) under the control of the BcMYBI promoter was constructed and transformed into tobacco (W38). And the assays of GUS activity confirmed that BcMYBI promoter could respond to drought stress to some extent in transgenic tobaccos. (C) 2004 Elsevier Ireland Ltd. All rights reserved.; Biochemistry & Molecular Biology; Plant Sciences; SCI(E); EI; 18; ARTICLE; 2; 493-500; 168
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/254486]
专题	生命科学学院
推荐引用方式 GB/T 7714	Chen, BJ,Wang, Y,Hu, YL,et al. Cloning and characterization of a drought-inducible MYB gene from Boea crassifolia[J]. plant science,2005.
APA	Chen, BJ,Wang, Y,Hu, YL,Wu, Q,&Lin, ZP.(2005).Cloning and characterization of a drought-inducible MYB gene from Boea crassifolia.plant science.
MLA	Chen, BJ,et al."Cloning and characterization of a drought-inducible MYB gene from Boea crassifolia".plant science (2005).