Nanog reporter system in mouse embryonic stem cells based on highly   efficient BAC homologous recombination

doi:10.1007/s11434-007-0435-2

CORC > 北京大学 > 生命科学学院

	Nanog reporter system in mouse embryonic stem cells based on highly efficient BAC homologous recombination
	Fan Yan ; Tong Zan ; You JieFang ; Du LiYing ; Zou FangDong ; Ding MingXiao ; Deng HongKui
刊名	科学通报英文版
	2007
关键词	mouse embryonic stem cells self-renewal Nanog EGFP bacterial artificial chromosome homologous recombination SELF-RENEWAL ES CELLS PLURIPOTENCY DIFFERENTIATION OCT4 EXPRESSION MAINTENANCE INDUCTION CARCINOMA CULTURE
DOI	10.1007/s11434-007-0435-2
英文摘要	Nanog is a novel transcription factor specifically expressed in mouse embryonic stem cells (mES cells). It has been reported that Nanog plays an essential role in maintaining multi-potency of ES cells. The expression of Nanog is very sensitive to ES cells differentiation, making Nanog one of the best markers to indicate the status of ES cells. In this study, we developed an efficient method to construct Nanog promoter driven EGFP reporter system based on the BAC homologous recombination. We further generated a Nanog-EGFP reporter mES cell line. This reporter mES cell line exhibited features similar to those of normal mES cells, and the EGFP reporter efficiently reflected the expression of Nanog, indicating the differentiation status of mES cells. We achieved a reliable experimental reporter system to research self-renewal and differentiation of mES cells. The system could facilitate research on culture system of mES cells and researches on the expression and regulation of Nanog and other related factors in mES cells.; Multidisciplinary Sciences; SCI(E); 0; ARTICLE; 20; 2782-2788; 52
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/252849]
专题	生命科学学院
推荐引用方式 GB/T 7714	Fan Yan,Tong Zan,You JieFang,et al. Nanog reporter system in mouse embryonic stem cells based on highly efficient BAC homologous recombination[J]. 科学通报英文版,2007.
APA	Fan Yan.,Tong Zan.,You JieFang.,Du LiYing.,Zou FangDong.,...&Deng HongKui.(2007).Nanog reporter system in mouse embryonic stem cells based on highly efficient BAC homologous recombination.科学通报英文版.
MLA	Fan Yan,et al."Nanog reporter system in mouse embryonic stem cells based on highly efficient BAC homologous recombination".科学通报英文版 (2007).