Construction of a non-antibiotic expression system in a marine   cyanobacterium Synechococcus sp PCC 7002 and its application in   production of oral vaccine against enterotoxin of Escherichia coli

doi:10.1007/s10811-006-9082-2

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	Construction of a non-antibiotic expression system in a marine cyanobacterium Synechococcus sp PCC 7002 and its application in production of oral vaccine against enterotoxin of Escherichia coli
	Xu, Dongyi ; Yu, Xin ; Guo, Cong ; Zhao, Jindong
刊名	journal of applied phycology
	2006
关键词	cyanobacteria LT-B non-antibiotic selection oral vaccine recombinant proteins HEAT-LABILE ENTEROTOXIN TOXIN-B-SUBUNIT CHOLERA-TOXIN ANABAENA PCC-7120 VIBRIO-CHOLERAE PHOTOSYSTEM-II GENE PROTEIN PLANTS CLONING
DOI	10.1007/s10811-006-9082-2
英文摘要	Synechococcus sp. PCC 7002 is a marine cyanobacterium that is rich in thylakoid membranes and performs oxygenic photosynthesis. In this study, we inactivated its psbEF genes encoding cytochrome b-559 of photosystem II and the mutant strain EF211 could not grow photoautotrophically. A non-antibiotic plasmid (pAQEF559) was constructed in such a way that it has a psbEF operon and all antibiotic genes are deleted. The plasmid could complement EF211 and restore photoautotrophical growth. This non-antibiotic plasmid was used to express the gene encoding heat-labile enterotoxin B-subunit, LT-B. The overproduced LT-B in the cyanobacterium could form pentamers and was associated with thylakoid membranes. Oral immunization of mice with the cyanobacterium overproducing LT-B showed that it was very effective in stimulating production of both IgG in serum and IgA in intestine. Our results demonstrate that the non-antibiotic vector in combination with the constructed host cyanobacterium is useful in production of recombinant proteins in cyanobacteria and the cyanobacterial cells producing LT-B can be an effective oral vaccine against enterotoxins.; Biotechnology & Applied Microbiology; Marine & Freshwater Biology; SCI(E); 1; ARTICLE; 2; 127-134; 18
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/252527]
专题	生命科学学院
推荐引用方式 GB/T 7714	Xu, Dongyi,Yu, Xin,Guo, Cong,et al. Construction of a non-antibiotic expression system in a marine cyanobacterium Synechococcus sp PCC 7002 and its application in production of oral vaccine against enterotoxin of Escherichia coli[J]. journal of applied phycology,2006.
APA	Xu, Dongyi,Yu, Xin,Guo, Cong,&Zhao, Jindong.(2006).Construction of a non-antibiotic expression system in a marine cyanobacterium Synechococcus sp PCC 7002 and its application in production of oral vaccine against enterotoxin of Escherichia coli.journal of applied phycology.
MLA	Xu, Dongyi,et al."Construction of a non-antibiotic expression system in a marine cyanobacterium Synechococcus sp PCC 7002 and its application in production of oral vaccine against enterotoxin of Escherichia coli".journal of applied phycology (2006).