Passage number affects the pluripotency of mouse embryonic stem cells as   judged by tetraploid embryo aggregation

doi:10.1007/s00441-006-0354-6

CORC > 北京大学 > 生命科学学院

	Passage number affects the pluripotency of mouse embryonic stem cells as judged by tetraploid embryo aggregation
	Li, Xiang-yun ; Jia, Qing ; Di, Ke-Qian ; Gao, Shu-min ; Wen, Xiao-hui ; Zhou, Rong-yan ; Wei, Wei ; Wang, Li-ze
刊名	cell and tissue research
	2007
关键词	embryonic stem cells tetraploid embryo aggregation pluripotency mouse IMPRINTED GENE METHYLATION FEMALE MICE ES CELLS COMPLEMENTATION DIFFERENTIATION ELECTROFUSION EXPRESSION GENERATION DERIVATION GENOME
DOI	10.1007/s00441-006-0354-6
英文摘要	The aim of this study was to determine whether the number of passages affected the developmental pluripotency of embryonic stem (ES) cells as measured by the attainment of adult fertile mice derived from embryonic stem (ES) cell/tetraploid embryo complementation. Thirty-six newborns were produced by the aggregation of tetraploid embryos and hybrid ES cells after various numbers of passages. These newborns were entirely derived from ES cells as judged by microsatellite DNA, coat-color phenotype, and germline transmission. Although 15 survived to adulthood, 17 died of respiratory failure, and four were eaten by their foster mother. From the 15 mice that reached adulthood and that could reproduce, none arose from ES cells at passage level 15 or more. All 15 arose from cells at passages 3-11. Our results demonstrate that the number of passages affects the developmental pluripotency of ES cells.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000244091100017&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Cell Biology; SCI(E); 17; ARTICLE; 3; 607-614; 327
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/252191]
专题	生命科学学院
推荐引用方式 GB/T 7714	Li, Xiang-yun,Jia, Qing,Di, Ke-Qian,et al. Passage number affects the pluripotency of mouse embryonic stem cells as judged by tetraploid embryo aggregation[J]. cell and tissue research,2007.
APA	Li, Xiang-yun.,Jia, Qing.,Di, Ke-Qian.,Gao, Shu-min.,Wen, Xiao-hui.,...&Wang, Li-ze.(2007).Passage number affects the pluripotency of mouse embryonic stem cells as judged by tetraploid embryo aggregation.cell and tissue research.
MLA	Li, Xiang-yun,et al."Passage number affects the pluripotency of mouse embryonic stem cells as judged by tetraploid embryo aggregation".cell and tissue research (2007).