Transcriptome analysis of Sinorhizobium meliloti nodule bacteria in nifA   mutant background

doi:10.1007/s11434-006-2092-2

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	Transcriptome analysis of Sinorhizobium meliloti nodule bacteria in nifA mutant background
	Tian Zhexian ; Zou Huasong ; Li Jian ; Zhang Yuantao ; Liu Ying ; Yu Guanqiao ; Zhu Jiabi ; Ruberg, Silvia ; Becker Anke ; Wang Yiping
刊名	科学通报英文版
	2006
关键词	transcriptorne NifA FixJ nodule bacteria ALFALFA ROOT-NODULE DNA-BINDING SITES RHIZOBIUM-MELILOTI NITROGEN-FIXATION GENE-EXPRESSION ESCHERICHIA-COLI REGULATORY PROTEINS OXYGEN SENSOR FIX MUTANTS ACTIVATOR
DOI	10.1007/s11434-006-2092-2
英文摘要	Gene expression profiles of a Sinorhizobium meliloti 1021 nifA mutant and wild type nodule bacteria were compared using whole genome microarrays. The results revealed a large scale alteration of gene expression (601 genes) in the nifA minus background. The loss of NifA altered the expression of many functional groups of genes (macromolecular metabolism, TCA cycle and respiration, nodulation and nitrogen fixation) and may lead to quite different life stages of the nodule bacteria. Upregulation of fixK and its associated genes was observed in the nifA mutant nodule bacteria. Additional quantitative real-time PCR experiments revealed that the transcript levels of fixLJ were significantly upshifted in the nifA mutant nodule bacteria. Putative NifA binding sites were predicted by a statistical method in the upstream sequences of 13 differentially regulated genes from the nifA transcriptome.; Multidisciplinary Sciences; SCI(E); 0; ARTICLE; 17; 2079-2086; 51
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/251615]
专题	生命科学学院
推荐引用方式 GB/T 7714	Tian Zhexian,Zou Huasong,Li Jian,et al. Transcriptome analysis of Sinorhizobium meliloti nodule bacteria in nifA mutant background[J]. 科学通报英文版,2006.
APA	Tian Zhexian.,Zou Huasong.,Li Jian.,Zhang Yuantao.,Liu Ying.,...&Wang Yiping.(2006).Transcriptome analysis of Sinorhizobium meliloti nodule bacteria in nifA mutant background.科学通报英文版.
MLA	Tian Zhexian,et al."Transcriptome analysis of Sinorhizobium meliloti nodule bacteria in nifA mutant background".科学通报英文版 (2006).