Complementation analyses of Sinorhizobium meliloti nifA mutant with   different originated nifA genes

doi:10.1007/s11434-006-2203-0

CORC > 北京大学 > 生命科学学院

	Complementation analyses of Sinorhizobium meliloti nifA mutant with different originated nifA genes
	Yao Zhenhua ; Tian Zhexian ; Dai Xiaomi ; Becker, Anke ; Li Jian ; Yan Haiqin ; Xiao Yan ; Zhu Jiabi ; Yu Guanqiao ; Rueverg, Silvia ; Wang Yiping ; Zou Huasong
刊名	科学通报英文版
	2006
关键词	Sinorhizobium meliloti NifA gene expression microarray RHIZOBIUM-LEGUME SYMBIOSIS NITROGEN-FIXATION NODULE EXPRESSION
DOI	10.1007/s11434-006-2203-0
英文摘要	A previous work inferred that the nifA gene of Enterobacter cloacae did not restore the symbiotic phenotype of Sinorhizobium meliloti nifA mutant. In the present study, two nifA genes of Bradyrhizobiurn japonicum and Mesorhizobium huakuii also did not restore the symbiotic phenotype of S. meliloti nifA mutant. In whole genomic microarray experiments, 238 genes were found to be differentially expressed after S. meliloti nifA had been constitutively expressed in its nifA mutant. In contrast, only 20, 7 and 9 genes changed their transcriptional levels when expressing B. japonium, M. huakuii and Enterobacter cloacae nifA genes in Sm nifA mutant separately. These genes were classified into severai functional groups including house keeping, energy and central intermediary metabolism, transport systems and symbiosis. Interestingly, the genes that of nifH operons showed high expression levels in the presence of either B. japonium or M. huakuii NifA, which was confirmed by subsequent lacZ fusion experiments.; Multidisciplinary Sciences; SCI(E); 2; ARTICLE; 22; 2748-2754; 51
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/251313]
专题	生命科学学院
推荐引用方式 GB/T 7714	Yao Zhenhua,Tian Zhexian,Dai Xiaomi,et al. Complementation analyses of Sinorhizobium meliloti nifA mutant with different originated nifA genes[J]. 科学通报英文版,2006.
APA	Yao Zhenhua.,Tian Zhexian.,Dai Xiaomi.,Becker, Anke.,Li Jian.,...&Zou Huasong.(2006).Complementation analyses of Sinorhizobium meliloti nifA mutant with different originated nifA genes.科学通报英文版.
MLA	Yao Zhenhua,et al."Complementation analyses of Sinorhizobium meliloti nifA mutant with different originated nifA genes".科学通报英文版 (2006).