Maize (Zea mays) genetic transformation by co-cultivating germinating   seeds with Agrobacterium tumefaciens

doi:10.1042/BA20060065

CORC > 北京大学 > 生命科学学院

	Maize (Zea mays) genetic transformation by co-cultivating germinating seeds with Agrobacterium tumefaciens
	Wang, Jingxue ; Sun, Yi ; Li, Yi
刊名	生物技术与应用生化学
	2007
关键词	Agrobacterium tumefaciens co-cultivation germinating seeds maize (Zea mays) FERTILE TRANSGENIC PLANTS EXPRESSION CELLS REGENERATION SYSTEM
DOI	10.1042/BA20060065
英文摘要	A novel plant genetic transformation method for maize (Zea mays) is reported. Using a scalpel, germinating seeds were wounded in the meristem and co-cultivated with an Agrobacterium tumefaciens strain harbouring a Ti plasmid. Seedlings produced from the treatment were screened by hygromycin selection. Fertile transgenic T-0 and T-1 plants were obtained. PCR amplification, PCR-Southern and Southern-blot analysis showed that the foreign gene had been introduced into the inbreds of maize. About 29% of T-0-seedlings examined were found to be transgenic, although the overall transformation was only 0.6% when total treated seeds were taken into account. The method circumvented tedious and prolonged tissue-culture steps, is simple and can be readily integrated into conventional plant breeding programs.; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; SCI(E); EI; 0; ARTICLE; 1; 51-55; 46
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/250759]
专题	生命科学学院
推荐引用方式 GB/T 7714	Wang, Jingxue,Sun, Yi,Li, Yi. Maize (Zea mays) genetic transformation by co-cultivating germinating seeds with Agrobacterium tumefaciens[J]. 生物技术与应用生化学,2007.
APA	Wang, Jingxue,Sun, Yi,&Li, Yi.(2007).Maize (Zea mays) genetic transformation by co-cultivating germinating seeds with Agrobacterium tumefaciens.生物技术与应用生化学.
MLA	Wang, Jingxue,et al."Maize (Zea mays) genetic transformation by co-cultivating germinating seeds with Agrobacterium tumefaciens".生物技术与应用生化学 (2007).