MexT modulates virulence determinants in Pseudomonas aeruginosa   independent of the MexEF-OprN efflux pump

doi:10.1016/j.micpath.2009.08.003

CORC > 北京大学 > 生命科学学院

	MexT modulates virulence determinants in Pseudomonas aeruginosa independent of the MexEF-OprN efflux pump
	Tian, Zhe-Xian ; Mac Aogain, Micheal ; O&apos ; Fargier, Emilie ; Mooij, Marlies J. ; Adams, Claire ; Wang, Yi-Ping ; O&apos ; Connor, Hazel F. ; Gara, Fergal
刊名	microbial pathogenesis
	2009
关键词	MexT Type three secretion Pseudomonas aeruginosa III SECRETION SYSTEM EXPRESSION OVEREXPRESSION REGULATOR BACTERIA GENES
DOI	10.1016/j.micpath.2009.08.003
英文摘要	In the human pathogen Pseudomonas aeruginosa, the LysR-family regulator MexT modulates the induction of the tripartite MexEF-OprN resistance nodulation-division multi-drug efflux system resulting in increased resistance to diverse antibiotics. The MexEF-OprN system is normally quiescent in wild-type cells, but is highly induced in nfxC-type phenotypic mutants in a MexT dependent manner. In addition to antibiotic resistance, induction of mexEF-oprN in nfxC-type mutants has been linked to reduced levels of homoserine lactone-dependent virulence traits, including pyocyanin, elastase, rhamnolipids and PQS and to reduced expression of type three secretion effector proteins. In this study, MexT is overexpressed in wild-type PAO1 and an isogenic mexEF deletion mutant to determine if MexT regulates diverse virulence phenotypes dependent or independent of MexEF-OprN. It is shown that MexT regulates type three secretion, pyocyanin production and early surface attachment independent of MexEF-OprN. In contrast, MexT modulation of the expression of the virulence genes rhlA, lasB and hcnB is dependent on MexEF-OprN, which apparently mediates these effects via efflux of cell-signaling intermediates. The data presented demonstrates that MexT may play a more global role in modulating P. aeruginosa virulence than previously reported and suggests that MexT regulates diverse targets that mediate phenotypic alterations independent of MexEF-OprN. (C) 2009 Elsevier Ltd. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000274533400007&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Immunology; Microbiology; SCI(E); 29; ARTICLE; 4; 237-241; 47
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/246225]
专题	生命科学学院
推荐引用方式 GB/T 7714	Tian, Zhe-Xian,Mac Aogain, Micheal,O&apos,et al. MexT modulates virulence determinants in Pseudomonas aeruginosa independent of the MexEF-OprN efflux pump[J]. microbial pathogenesis,2009.
APA	Tian, Zhe-Xian.,Mac Aogain, Micheal.,O&apos.,Fargier, Emilie.,Mooij, Marlies J..,...&Gara, Fergal.(2009).MexT modulates virulence determinants in Pseudomonas aeruginosa independent of the MexEF-OprN efflux pump.microbial pathogenesis.
MLA	Tian, Zhe-Xian,et al."MexT modulates virulence determinants in Pseudomonas aeruginosa independent of the MexEF-OprN efflux pump".microbial pathogenesis (2009).