生物素-亲和素放大酶联免疫吸附法测定氯胺酮

CORC > 北京大学 > 生命科学学院

	生物素-亲和素放大酶联免疫吸附法测定氯胺酮; Biotin-avidin Mediated Competitive Enzyme-linked Immunosorbent Assay for Determination of Ketamine
	张雯迪 ; 苏萍 ; 杨屹 ; 郭振泉
刊名	分析化学
	2010
关键词	氯胺酮单克隆抗体生物素-亲和素放大酶联免疫吸附法 Ketamine Monoclonal antibody Biotin-avidin mediated competitive enzyme-linked immunosor bent assay Ketamine Monoclonal antibody Biotin-avidin mediated competitive enzyme-linked immunosorbent assay
英文摘要	建立了检测氯胺酮的生物素-亲和素放大酶联免疫吸附测定法(BA-ELISA).实验最佳测定条件:抗原包被浓度为2.0 mg/L、氯胺酮单克隆抗体浓度为10.2 mg/L,生物素化羊抗小鼠IgG(Biotin-Igc)和酶标链霉亲和素(SA-HRP)的最佳反应浓度分别为0.29和1.0 mg/L.在此优化条件下,方法的线性范围为0.1-1000μg/L;检出限为0.03 μg/L.氯胺酮生物样品的加标回收率为94%～102%.与酶标二抗体系ELISA法相比,BA-ELISA具有更高的灵敏度,适于低浓度氯胺酮的检测.; A rapid and sensitive method based on biotin-avidin mediated competitive enzyme-linked immunosorbent assay( BA-ELISA) was established for the determination of ketamine. The optimal concentration of coated antigen and anti-ketamine monoclonal antibody were found to be 2.0 and 10.2 mg/L. The concentrations of biotinylated goat anti-mouse IgG (Biotin-IgG) and streptavidin-horseradish peroxidase (SA-HRP) were optimized and the optimum results were found to be 0.29 and 1.0 mg/L, respectively. The linear range of the presented method was from 0.1 to 1000 mu g/L, and the limit of detection was found to be 0.03 mu g/L. The recoveries of ketamine spiked in human serum and urine were between 94% and 102%. Comparing the result of traditional ELISA, the present BA-ELISA method had a lower detection limit for ketamine. The experimental results indicated that the present BA-ELISA method was specific and sensitive.; 国家自然科学基金; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000274371500023&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; SCI(E); 中文核心期刊要目总览(PKU); 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 0; 1; 117-120; 38
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/245668]
专题	生命科学学院
推荐引用方式 GB/T 7714	张雯迪,苏萍,杨屹,等. 生物素-亲和素放大酶联免疫吸附法测定氯胺酮, Biotin-avidin Mediated Competitive Enzyme-linked Immunosorbent Assay for Determination of Ketamine[J]. 分析化学,2010.
APA	张雯迪,苏萍,杨屹,&郭振泉.(2010).生物素-亲和素放大酶联免疫吸附法测定氯胺酮.分析化学.
MLA	张雯迪,et al."生物素-亲和素放大酶联免疫吸附法测定氯胺酮".分析化学 (2010).