A new cotton SDR family gene encodes a polypeptide possessing aldehyde   reductase and 3-ketoacyl-CoA reductase activities

doi:10.1134/S0006297910030089

CORC > 北京大学 > 生命科学学院

	A new cotton SDR family gene encodes a polypeptide possessing aldehyde reductase and 3-ketoacyl-CoA reductase activities
	Pang, Yu ; Song, Wen-Qiang ; Chen, Fang-Yuan ; Qin, Yong-Mei
刊名	biochemistry moscow
	2010
关键词	Gossypium hirsutum short-chain alcohol dehydrogenase/reductase family 3-ketoacyl-CoA reductase fatty acid elongation very long-chain fatty acids FATTY-ACID ELONGASE CELL ELONGATION ENDOPLASMIC-RETICULUM CUTICULAR WAXES COMPONENT FIBER PROTEINS BIOSYNTHESIS BIOGENESIS METABOLISM
DOI	10.1134/S0006297910030089
英文摘要	To understand regulatory mechanisms of cotton fiber development, microarray analysis has been performed for upland cotton (Gossypium hirsutum). Based on this, a cDNA (GhKCR3) encoding a polypeptide belonging to short-chain alcohol dehydrogenase/reductase family was isolated and cloned. It contains an open reading frame of 987 bp encoding a polypeptide of 328 amino acid residues. Following its overexpression in bacterial cells, the purified recombinant protein specifically uses NADPH to reduce a variety of short-chain aldehydes. A fragment between Gly180 and Gly191 was found to be essential for its catalytic activity. Though the GhKCR3 gene shares low sequence similarities to the ortholog of Saccharomyces cerevisiae YBR159w that encodes 3-ketoacyl-CoA reductase (KCR) catalyzing the second step of fatty acid elongation, it was surprisingly able to complement the yeast ybr159w delta mutant. Gas chromatography-mass spectrometry analysis showed that very long-chain fatty acids, especially C26:0, were produced in the ybr159w delta mutant cells expressing GhKCR3. Applying palmitoyl-CoA and malonyl-CoA as substrates, GhKCR3 showed KCR activity in vitro. Quantitative real time-PCR analysis indicated GhKCR3 transcripts accumulated in rapidly elongating fibers, roots, and stems. Our results suggest that GhKCR3 is probably a novel KCR contributing to very long-chain fatty acid biosynthesis in plants.; Biochemistry & Molecular Biology; SCI(E); EI; 2; 3; 320-326; 75
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/244314]
专题	生命科学学院
推荐引用方式 GB/T 7714	Pang, Yu,Song, Wen-Qiang,Chen, Fang-Yuan,et al. A new cotton SDR family gene encodes a polypeptide possessing aldehyde reductase and 3-ketoacyl-CoA reductase activities[J]. biochemistry moscow,2010.
APA	Pang, Yu,Song, Wen-Qiang,Chen, Fang-Yuan,&Qin, Yong-Mei.(2010).A new cotton SDR family gene encodes a polypeptide possessing aldehyde reductase and 3-ketoacyl-CoA reductase activities.biochemistry moscow.
MLA	Pang, Yu,et al."A new cotton SDR family gene encodes a polypeptide possessing aldehyde reductase and 3-ketoacyl-CoA reductase activities".biochemistry moscow (2010).