Up-Regulation of Licochalcone A Biosynthesis and Secretion by Tween 80   in Hairy Root Cultures of Glycyrrhiza uralensis Fisch

doi:10.1007/s12033-010-9311-4

CORC > 北京大学 > 生命科学学院

	Up-Regulation of Licochalcone A Biosynthesis and Secretion by Tween 80 in Hairy Root Cultures of Glycyrrhiza uralensis Fisch
	Zhang, Hai-Chao ; Liu, Jing-Mei ; Chen, Hai-Min ; Gao, Chun-Chun ; Lu, Hong-Yu ; Zhou, Hua ; Li, Yi ; Gao, Shan-Lin
刊名	molecular biotechnology
	2011
关键词	Elicitor Flavonoids Glycyrrhiza uralensis Fisch Hairy roots Licochalcone A Tween 80 CINNAMIC ACID 4-HYDROXYLASE STORED TROPANE ALKALOIDS AMMONIA-LYASE ACTIVITY SECONDARY METABOLITES SUSPENSION-CULTURES EXPRESSION RELEASE ARABIDOPSIS ELICITOR LICORICE
DOI	10.1007/s12033-010-9311-4
英文摘要	We evaluated the effect of Tween 80 as elicitor on licochalcone A from hairy root cultures of Glycyrrhiza uralensis Fisch. After a 15-days treatment with 2% Tween 80, hairy roots still grew well and produced higher levels of licochalcone A and total flavonoids than the control (without treatment). Licochalcone A content and total flavonoid content were 3.103 and 127.095 mg per flask (9- and 11-fold higher), respectively, compared with controls. Secretion of licochalcone A and total flavonoids into the culture medium was remarkably high, up to 98 and 94% of the total production, respectively. The enhanced flavonoid production was associated with elevated mRNA levels and enzyme activities of phenylalanine ammonia-lyase (PAL), 4-coumarate:coenzyme A ligase (4CL), and cinnamate-4-hydroxylase (C4H). These results clearly demonstrated that Tween 80 treatment permeabilized the roots to enhance secretion, but also acted as an efficient elicitor of licochalcone A and total flavonoid production in hairy roots of G. uralensis Fisch.; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; SCI(E); EI; 9; ARTICLE; 1; 50-56; 47
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/241214]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zhang, Hai-Chao,Liu, Jing-Mei,Chen, Hai-Min,et al. Up-Regulation of Licochalcone A Biosynthesis and Secretion by Tween 80 in Hairy Root Cultures of Glycyrrhiza uralensis Fisch[J]. molecular biotechnology,2011.
APA	Zhang, Hai-Chao.,Liu, Jing-Mei.,Chen, Hai-Min.,Gao, Chun-Chun.,Lu, Hong-Yu.,...&Gao, Shan-Lin.(2011).Up-Regulation of Licochalcone A Biosynthesis and Secretion by Tween 80 in Hairy Root Cultures of Glycyrrhiza uralensis Fisch.molecular biotechnology.
MLA	Zhang, Hai-Chao,et al."Up-Regulation of Licochalcone A Biosynthesis and Secretion by Tween 80 in Hairy Root Cultures of Glycyrrhiza uralensis Fisch".molecular biotechnology (2011).