miR-764-5p promotes osteoblast differentiation through inhibition of   CHIP/STUB1 expression

doi:10.1002/jbmr.1597

CORC > 北京大学 > 生命科学学院

	miR-764-5p promotes osteoblast differentiation through inhibition of CHIP/STUB1 expression
	Guo, Junwei ; Ren, Fangli ; Wang, Yinyin ; Li, Shan ; Gao, Zhengrong ; Wang, Xiaoyan ; Ning, Hongxiu ; Wu, Jianguo ; Li, Yi ; Wang, Zhao ; Chim, Shek Man ; Xu, Jiake ; Chang, Zhijie
刊名	journal of bone and mineral research
	2012
关键词	miR-764-5p miRNA CHIP RUNX2 OSTEOBLAST DIFFERENTIATION HUMAN MICRORNA TARGETS STEM-CELLS PROTEIN CHIP DEGRADATION LIGASE BONE IDENTIFICATION RNAS COMPLEXES
DOI	10.1002/jbmr.1597
英文摘要	Differentiation of committed precursor cells into the osteoblast lineage is tightly regulated by several factors, including Runx2 and BMP2. We previously reported that C terminus of Hsc70-interacting protein/STIP1 homology and U-Box containing protein 1 (CHIP/STUB1) negatively regulated osteoblast differentiation through promoting Runx2 protein degradation. However, how CHIP is regulated during osteoblast differentiation remains unknown. In this study, we found that miR-764-5p is up-expressed during the osteoblast differentiation in calvarial and osteoblast progenitor cells, coupled with down-expression of CHIP protein. We observed that forced expression or inhibition of miR-764-5p decreased or increased the CHIP protein level through affecting its translation by targeting the 3'-UTR region. Perturbation of miR-764-5p resulted in altered differentiation fate of osteoblast progenitor cells and the role of miR-764-5p was reversed by overexpression of CHIP, whereas depletion of CHIP impaired the effect of miR-764-5p. Our data showed that miR-764-5p positively regulates osteoblast differentiation from osteoblast progenitor cells by repressing the translation of CHIP protein. (c) 2012 American Society for Bone and Mineral Research.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000305297000020&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Endocrinology & Metabolism; SCI(E); 21; ARTICLE; 7; 1607-1618; 27
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/232555]
专题	生命科学学院
推荐引用方式 GB/T 7714	Guo, Junwei,Ren, Fangli,Wang, Yinyin,et al. miR-764-5p promotes osteoblast differentiation through inhibition of CHIP/STUB1 expression[J]. journal of bone and mineral research,2012.
APA	Guo, Junwei.,Ren, Fangli.,Wang, Yinyin.,Li, Shan.,Gao, Zhengrong.,...&Chang, Zhijie.(2012).miR-764-5p promotes osteoblast differentiation through inhibition of CHIP/STUB1 expression.journal of bone and mineral research.
MLA	Guo, Junwei,et al."miR-764-5p promotes osteoblast differentiation through inhibition of CHIP/STUB1 expression".journal of bone and mineral research (2012).