Induced apoptosis of osteoblasts proliferating on polyhydroxyalkanoates

doi:10.1016/j.biomaterials.2013.01.088

CORC > 北京大学 > 生命科学学院

	Induced apoptosis of osteoblasts proliferating on polyhydroxyalkanoates
	Wang, Yang ; Jiang, Xian-Li ; Peng, Si-Wu ; Guo, Xiao-Yong ; Shang, Guan-Guan ; Chen, Jin-Chun ; Wu, Qiong ; Chen, Guo-Qiang
刊名	biomaterials
	2013
关键词	Polyhydroxyalkanoates Osteoblast Apoptosis Proliferation Integrin Caspase-8 FOCAL ADHESION KINASE PROTEIN ADSORPTION CELL-ADHESION SURFACE-ROUGHNESS STEM-CELLS INTEGRINS FIBROBLAST CYCLE ALPHA-V-BETA-3 TERPOLYESTER
DOI	10.1016/j.biomaterials.2013.01.088
英文摘要	The mechanism study on behaviors of cells influenced by biomaterial surface properties can provide profound guidances for functional tissue engineering scaffolds design. In this study, regulation of integrin-mediated cell substrate interactions using rat osteoblasts incubated on PHA films was investigated. Compared with tissue culture plate (TCP), poly-3-hydroxybutyrate (PHB), copolymer of 3-hydroxybutyrate and 3-hydroxyvalerate (PHBV) and copolymer of 3-hydroxybutyrate and 3-hydroxyhexanoate (PHBHHx), osteoblasts inoculated on a terpolymer of 3-hydroxybutyrate, 3-hydroxyvalerate and 3-hydroxyhexanoate (PHBVHHx) were found to have higher apoptosis rates. Several integrin subunits in osteoblasts grown on PHBVHHx showed altered expressions. Simultaneously, extracellular matrics (ECM) were also remodeled on the material surface. Osteoblasts showed a higher expression of integrin subunit beta 3 and ay on PHBVHHx films compared with that on TCP. On the other hand, less vitronectin, osteopontin and fibronectin, the main ligands for integrin beta 3 were expressed and deposited in ECM. The unligated integrin beta 3 could recruit caspase-8 to the membrane and activate its downstream signaling which was proven by the caspase-8 activation assay. It was therefore concluded that the induced apoptosis of osteoblasts on PHBVHHx was regulated by recruitment of caspase-8 to the unligated integrin beta 3. (C) 2013 Elsevier Ltd. All rights reserved.; Engineering, Biomedical; Materials Science, Biomaterials; SCI(E); EI; 2; ARTICLE; 15; 3737-3746; 34
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/224314]
专题	生命科学学院
推荐引用方式 GB/T 7714	Wang, Yang,Jiang, Xian-Li,Peng, Si-Wu,et al. Induced apoptosis of osteoblasts proliferating on polyhydroxyalkanoates[J]. biomaterials,2013.
APA	Wang, Yang.,Jiang, Xian-Li.,Peng, Si-Wu.,Guo, Xiao-Yong.,Shang, Guan-Guan.,...&Chen, Guo-Qiang.(2013).Induced apoptosis of osteoblasts proliferating on polyhydroxyalkanoates.biomaterials.
MLA	Wang, Yang,et al."Induced apoptosis of osteoblasts proliferating on polyhydroxyalkanoates".biomaterials (2013).