Site-specific antibody of (Na++K+)-ATPase augments cardiac myocyte   contraction without inactivating enzyme activity

doi:10.1006/bbrc.2001.5974

CORC > 北京大学 > 生命科学学院

	Site-specific antibody of (Na++K+)-ATPase augments cardiac myocyte contraction without inactivating enzyme activity
	Xu, KY ; Wang, SQ ; Cheng, HP
刊名	生物化学与生物物理学研究通讯
	2001
关键词	(Na+ + K+)-ATPase site-specific antibody Ca2+ transients positive inotropic effect digitalis glycosides SODIUM-PUMP ADENOSINE-TRIPHOSPHATASE MOLECULAR-CLONING CATALYTIC SUBUNIT ALPHA-SUBUNIT RAT-BRAIN NA+,K+-ATPASE NA,K-ATPASE BINDING POLYPEPTIDE
DOI	10.1006/bbrc.2001.5974
英文摘要	(Na+ + K+)-ATPase regulates both excitability and contractility of the heart. Little is known about the molecular basis of the enzyme that underlies its cardiac regulatory functions. Here we demonstrate that the (833)KRQPRNPKTDKLVNE(847), region, which resides in the a-subunit of rat (Na+ + K+)-ATPase, directly participates in the regulation of cardiac contraction. A site-specific antibody (SSA95) against this peptide sequence markedly increased intracellular Ca2+ transients and contraction (EC50 = 11.4 nM) in intact rat heart cells without inactivating the (Na+ + K+)-ATPase. These novel findings establish the first link between a precise structural region of the (Na+ + K+)-ATPase and cardiac positive inotropy. (C) 2001 Academic Press.; Biochemistry & Molecular Biology; Biophysics; SCI(E); 5; ARTICLE; 1; 167-172; 289
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/210622]
专题	生命科学学院
推荐引用方式 GB/T 7714	Xu, KY,Wang, SQ,Cheng, HP. Site-specific antibody of (Na++K+)-ATPase augments cardiac myocyte contraction without inactivating enzyme activity[J]. 生物化学与生物物理学研究通讯,2001.
APA	Xu, KY,Wang, SQ,&Cheng, HP.(2001).Site-specific antibody of (Na++K+)-ATPase augments cardiac myocyte contraction without inactivating enzyme activity.生物化学与生物物理学研究通讯.
MLA	Xu, KY,et al."Site-specific antibody of (Na++K+)-ATPase augments cardiac myocyte contraction without inactivating enzyme activity".生物化学与生物物理学研究通讯 (2001).