Site-specific antibody of (Na++K+)-ATPase augments cardiac myocyte contraction without inactivating enzyme activity | |
Xu, KY ; Wang, SQ ; Cheng, HP | |
刊名 | 生物化学与生物物理学研究通讯
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2001 | |
关键词 | (Na+ + K+)-ATPase site-specific antibody Ca2+ transients positive inotropic effect digitalis glycosides SODIUM-PUMP ADENOSINE-TRIPHOSPHATASE MOLECULAR-CLONING CATALYTIC SUBUNIT ALPHA-SUBUNIT RAT-BRAIN NA+,K+-ATPASE NA,K-ATPASE BINDING POLYPEPTIDE |
DOI | 10.1006/bbrc.2001.5974 |
英文摘要 | (Na+ + K+)-ATPase regulates both excitability and contractility of the heart. Little is known about the molecular basis of the enzyme that underlies its cardiac regulatory functions. Here we demonstrate that the (833)KRQPRNPKTDKLVNE(847), region, which resides in the a-subunit of rat (Na+ + K+)-ATPase, directly participates in the regulation of cardiac contraction. A site-specific antibody (SSA95) against this peptide sequence markedly increased intracellular Ca2+ transients and contraction (EC50 = 11.4 nM) in intact rat heart cells without inactivating the (Na+ + K+)-ATPase. These novel findings establish the first link between a precise structural region of the (Na+ + K+)-ATPase and cardiac positive inotropy. (C) 2001 Academic Press.; Biochemistry & Molecular Biology; Biophysics; SCI(E); 5; ARTICLE; 1; 167-172; 289 |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.pku.edu.cn/handle/20.500.11897/210622] ![]() |
专题 | 生命科学学院 |
推荐引用方式 GB/T 7714 | Xu, KY,Wang, SQ,Cheng, HP. Site-specific antibody of (Na++K+)-ATPase augments cardiac myocyte contraction without inactivating enzyme activity[J]. 生物化学与生物物理学研究通讯,2001. |
APA | Xu, KY,Wang, SQ,&Cheng, HP.(2001).Site-specific antibody of (Na++K+)-ATPase augments cardiac myocyte contraction without inactivating enzyme activity.生物化学与生物物理学研究通讯. |
MLA | Xu, KY,et al."Site-specific antibody of (Na++K+)-ATPase augments cardiac myocyte contraction without inactivating enzyme activity".生物化学与生物物理学研究通讯 (2001). |
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