Gene therapy for diabetic rats by electroporational transfer of naked   plasmid with human pre-pro-insulin gene into skeletal muscle

doi:10.1023/A:1015506822817

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	Gene therapy for diabetic rats by electroporational transfer of naked plasmid with human pre-pro-insulin gene into skeletal muscle
	Yang, JC ; Liu, J ; Yang, XW ; Tang, JG
刊名	biotechnology letters
	2002
关键词	blood glucose diabetes mellitus electroporation gene therapy insulin-like protein IN-VIVO ADENOASSOCIATED VIRUS EXPRESSION VECTORS MICE EFFICIENCY CELLS
DOI	10.1023/A:1015506822817
英文摘要	A naked plasmid with human pre-pro-insulin gene was transferred into skeletal muscle of diabetic rats by electric pulses and gene expression was detected. Blood glucose concentration was decreased from 24 mmol l(-1) to 8.5 mmol l(-1). Circulating insulin-like protein was increased significantly to 15-20 muU ml(-1), while that of the control group injected with the empty vector remained at less than 10 muU ml(-1). The low blood glucose concentration lasted for more than two months. These studies indicate that electroporational transfer of plasmid with human pre-pro-insulin gene into skeletal muscle could be a potential method of gene therapy for human diabetes mellitus.; Biotechnology & Applied Microbiology; SCI(E); 5; ARTICLE; 10; 851-855; 24
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/208955]
专题	生命科学学院
推荐引用方式 GB/T 7714	Yang, JC,Liu, J,Yang, XW,et al. Gene therapy for diabetic rats by electroporational transfer of naked plasmid with human pre-pro-insulin gene into skeletal muscle[J]. biotechnology letters,2002.
APA	Yang, JC,Liu, J,Yang, XW,&Tang, JG.(2002).Gene therapy for diabetic rats by electroporational transfer of naked plasmid with human pre-pro-insulin gene into skeletal muscle.biotechnology letters.
MLA	Yang, JC,et al."Gene therapy for diabetic rats by electroporational transfer of naked plasmid with human pre-pro-insulin gene into skeletal muscle".biotechnology letters (2002).