Sustained PDGF-BB release from PHBHHx loaded nanoparticles in 3D   hydrogel/stem cell model

doi:10.1002/jbm.a.35149

CORC > 北京大学 > 生命科学学院

	Sustained PDGF-BB release from PHBHHx loaded nanoparticles in 3D hydrogel/stem cell model
	Dong, Cui-Ling ; Webb, William R. ; Peng, Qiang ; Tang, James Z. ; Forsyth, Nicholas R. ; Chen, Guo-Qiang ; El Haj, Alicia J.
刊名	journal of biomedical materials research part a
	2015
关键词	PHB polyhydroxyalkanoates nanoparticles platelet-derived growth factor mesenchymal stem cell tissue engineering MESENCHYMAL STEM-CELLS FIBROBLAST-GROWTH-FACTOR BIODEGRADABLE NANOPARTICLES PHOSPHOLIPID COMPLEX ORAL DELIVERY BONE-MARROW INSULIN TISSUE POLYHYDROXYALKANOATES DIFFERENTIATION
DOI	10.1002/jbm.a.35149
英文摘要	This study aimed to design a growth factor loaded copolyester of 3-hydroxybutyrate and 3-hydroxyhexanoate (PHBHHx) nanoparticles containing 3D collagen matrix to achieve growth factor sustained release for long-term stimulation of human mesenchymal stem cells (hMSCs) proliferation/differentiation for tissue engineer application. Platelet-derived growth factor-BB (PDGF-BB), which is known to enhance hMSCs proliferation in human serum, was selected as a model growth factor, and biodegradable copolyester of PHBHHx was chosen to be the sustained release vehicle. PDGF-BB phospholipid complex encapsulated PHBHHx nanoparticles were fabricated, and their effect on hMSCs proliferation was investigated via assays of CCK-8 and live-dead staining to cells inoculated in 2D tissue culture plates and 3D collagen gel scaffolds, respectively. The resulting spherical PHBHHx nanoparticles were stable in terms of their mean particle size, polydispersity index and zeta potential before and after lyophilization. In vitro study revealed a sustained release of PDGF-BB with a low burst release. Furthermore, sustained released PDGF-BB was revealed to significantly promote hMSCs proliferation in both cell monolayer and cell seeded 3D collagen scaffolds inoculated in serum-free media. Therefore, the 3D collagen matrices with locally sustained release growth factor nanoparticles hold promise to be used for stem cell tissue engineering. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 282-288, 2015.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000345572100032&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Engineering, Biomedical; Materials Science, Biomaterials; SCI(E); 1; ARTICLE; chengq@mail.tsinghua.edu.cn; a.j.el.haj@keele.ac.uk; 1; 282-288; 103
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/206748]
专题	生命科学学院
推荐引用方式 GB/T 7714	Dong, Cui-Ling,Webb, William R.,Peng, Qiang,et al. Sustained PDGF-BB release from PHBHHx loaded nanoparticles in 3D hydrogel/stem cell model[J]. journal of biomedical materials research part a,2015.
APA	Dong, Cui-Ling.,Webb, William R..,Peng, Qiang.,Tang, James Z..,Forsyth, Nicholas R..,...&El Haj, Alicia J..(2015).Sustained PDGF-BB release from PHBHHx loaded nanoparticles in 3D hydrogel/stem cell model.journal of biomedical materials research part a.
MLA	Dong, Cui-Ling,et al."Sustained PDGF-BB release from PHBHHx loaded nanoparticles in 3D hydrogel/stem cell model".journal of biomedical materials research part a (2015).