Comparative proteome analysis of human temporal cortex lobes by   two-dimensional electrophoresis and identification of selected common   proteins

doi:10.1023/A:1020383313177

CORC > 北京大学 > 生命科学学院

	Comparative proteome analysis of human temporal cortex lobes by two-dimensional electrophoresis and identification of selected common proteins
	Chen, W ; Ji, JG ; Zhao, R ; Ru, BG
刊名	神经化学研究
	2002
关键词	proteome comparison identification human temporal lobe two-dimensional gel electrophoresis mass spectrometry LASER DESORPTION IONIZATION IMMOBILIZED PH GRADIENTS MASS-SPECTROMETRY HUMAN GENOME SEQUENCE PROGRESS
DOI	10.1023/A:1020383313177
英文摘要	Human brain proteins were isolated from left and right temporal cortex lobes at the age of 73, 23, 84 years and separated by two-dimensional gel electrophoresis (2-DE). 2-DE was carried out with an immobilized pH gradient strip in the first dimension and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the second dimension. Over 800 polypeptide spots were resolved with a silver-staining protocol by computerized 2-D gel analsis. Seven of the polypeptide spots were evidently distinguishable between human left and right temporal lobes. Four of the polypeptide spots were larger and three were smaller in human right temporal lobe. One of these three protein spots that have descendent expression in human right temporal lobe was identified as carbonyl reductase (NADPH) 1 by MALDI-TOF MS. Thirty-three common spots were identified by ESI-MS/MALDI-TOF MS/Edman sequencing and a protein database search. These identified proteins include some important enzymes and regulating proteins.; Biochemistry & Molecular Biology; Neurosciences; SCI(E); PubMed; 10; ARTICLE; 9; 871-881; 27
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/200404]
专题	生命科学学院
推荐引用方式 GB/T 7714	Chen, W,Ji, JG,Zhao, R,et al. Comparative proteome analysis of human temporal cortex lobes by two-dimensional electrophoresis and identification of selected common proteins[J]. 神经化学研究,2002.
APA	Chen, W,Ji, JG,Zhao, R,&Ru, BG.(2002).Comparative proteome analysis of human temporal cortex lobes by two-dimensional electrophoresis and identification of selected common proteins.神经化学研究.
MLA	Chen, W,et al."Comparative proteome analysis of human temporal cortex lobes by two-dimensional electrophoresis and identification of selected common proteins".神经化学研究 (2002).