Functional properties of a recombinant chimeric plasminogen activator   with platelet-targeted fibrinolytic and anticoagulant potential

doi:10.1016/j.ymgme.2004.05.008

CORC > 北京大学 > 生命科学学院

	Functional properties of a recombinant chimeric plasminogen activator with platelet-targeted fibrinolytic and anticoagulant potential
	Zhang, LL ; Wang, J ; Yu, MM ; Ru, BG
刊名	分子遗传学与新陈代谢
	2004
关键词	decorsin hirudin platelet-targeted antithrombin activity RGD-PROTEIN DECORSIN PRO-UROKINASE THROMBIN INHIBITOR AGGREGATION BINDING PLASMA
DOI	10.1016/j.ymgme.2004.05.008
英文摘要	The construction, purification, and characterization of dscuPA33khC, a bifunctional protein designed for thrombosis treatment is described. The chimera was designed to consist of a decorsin (platelet aggregation inhibitor), a low molecular mass (33 kDa) single-chain urokinase (scuPA-33k), and a thrombin inhibitory domain. We have successfully produced this recombinant protein in the Escherichia coli expression system, in which the target protein exists in the form of inclusion bodies. After refolding by dilution in vitro, the chimeric protein was purified to homogeneity by immobilized metal affinity chromatography, ion-exchange chromatography, and gel filtration chromatography. The dscuPA33khC could directly activate plasminogen following Michaelis-Menten kinetics with K-m = 1.52 M and K-2 = 0.0024 s(-) (1). The specific activity of the chimera detected by fibrin plate determination was 11,000 IU/mg, which suggested a high thrombolysis effect. However, the chimeric dscuPA33khC bound the activated platelet and significantly increased affinity to platelet clots as compared to fibrin clots. It was found to inhibit ADP-induced platelet aggregation in a concentration-dependent manner as well as it exhibits antithrombin activity. These results suggest that the chimeric protein not only has platelet-targeted thrombolytic activity but also obtains anti-thrombus function. (C) 2004 Elsevier Inc. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000223570900005&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; Genetics & Heredity; Medicine, Research & Experimental; SCI(E); PubMed; 8; ARTICLE; 4; 304-311; 82
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/200273]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zhang, LL,Wang, J,Yu, MM,et al. Functional properties of a recombinant chimeric plasminogen activator with platelet-targeted fibrinolytic and anticoagulant potential[J]. 分子遗传学与新陈代谢,2004.
APA	Zhang, LL,Wang, J,Yu, MM,&Ru, BG.(2004).Functional properties of a recombinant chimeric plasminogen activator with platelet-targeted fibrinolytic and anticoagulant potential.分子遗传学与新陈代谢.
MLA	Zhang, LL,et al."Functional properties of a recombinant chimeric plasminogen activator with platelet-targeted fibrinolytic and anticoagulant potential".分子遗传学与新陈代谢 (2004).