Sulfonation of polyvinylidene difluoride resin and its application in   extraction of restriction enzymes from DNA digestion solutions

doi:10.1016/S0003-2697(03)00463-9

CORC > 北京大学 > 生命科学学院

	Sulfonation of polyvinylidene difluoride resin and its application in extraction of restriction enzymes from DNA digestion solutions
	Yang, J ; Dong, CX ; Huang, X ; Zhao, JD
刊名	分析生物化学
	2003
关键词	DNA digestion enzyme extraction PVDF sulfonated PVDF restriction enzymes BINDING PROTEINS PURIFICATION MEMBRANES
DOI	10.1016/S0003-2697(03)00463-9
英文摘要	Sulfonation of polyvinylidene difluoride (PVDF) resin was achieved by incubation of the resin with sulfuric acid at a moderately high temperature. The sulfonated PVDF (SPVDF) resin was studied for its ability to extract restriction enzymes from DNA digestion solutions. The SPVDF resin was effective in adsorbing restriction enzymes such as EcoRI and BamHI and the extraction procedure was easy and simple to perform. The adsorption depended upon the amount of the resin added. We found that 1 mg of the SPVDF resin could completely remove all restriction enzyme activity routinely used in DNA digestion within 2 min after its addition. Treatment of a digestion solution with the SPVDF resin did not change the reaction solution and the same digestion buffer could be used for another digestion of the same DNA with other enzymes. We also found that, in comparison with normal PVDF, the SPVDF resin adsorbed less DNA, resulting in less loss of DNA in the extraction step. The potential application of the SPVDF resin in other procedures of molecular cloning and enzyme purification is discussed. (C) 2003 Elsevier Inc. All rights reserved.; Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Analytical; SCI(E); PubMed; 2; ARTICLE; 1; 99-103; 322
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/200028]
专题	生命科学学院
推荐引用方式 GB/T 7714	Yang, J,Dong, CX,Huang, X,et al. Sulfonation of polyvinylidene difluoride resin and its application in extraction of restriction enzymes from DNA digestion solutions[J]. 分析生物化学,2003.
APA	Yang, J,Dong, CX,Huang, X,&Zhao, JD.(2003).Sulfonation of polyvinylidene difluoride resin and its application in extraction of restriction enzymes from DNA digestion solutions.分析生物化学.
MLA	Yang, J,et al."Sulfonation of polyvinylidene difluoride resin and its application in extraction of restriction enzymes from DNA digestion solutions".分析生物化学 (2003).