An Arabidopsis promoter microarray and its initial usage in the   identification of HY5 binding targets in vitro

doi:10.1023/B:PLAN.0000040898.86788.59

CORC > 北京大学 > 生命科学学院

	An Arabidopsis promoter microarray and its initial usage in the identification of HY5 binding targets in vitro
	Gao, Y ; Li, JM ; Strickland, E ; Hua, SJ ; Zhao, HY ; Chen, ZL ; Qu, LJ ; Deng, XW
刊名	植物分子生物学
	2004
关键词	Arabidopsis HY5 promoter microarray transcription factor-promoter interaction DNA-BINDING LIGHT CONTROL CIS-ELEMENTS ZINC FINGERS SACCHAROMYCES-CEREVISIAE CAENORHABDITIS-ELEGANS TRANSCRIPTION FACTORS REGULATORY ELEMENTS DROSOPHILA EMBRYO GENE-EXPRESSION
DOI	10.1023/B:PLAN.0000040898.86788.59
英文摘要	To analyze transcription factor-promoter interactions in Arabidopsis, a general strategy for generating a promoter microarray has been established. This includes an integrated platform for promoter sequence extraction and the design of primers for the PCR amplification of the promoter regions of annotated genes in the Arabidopsis genome. A web-interfaced primer-retrieval program was used to obtain up to 10 primer pairs with a suitability ranking given to each gene. We selected primer pairs for the promoters of about 3800 genes, and greater than 95% of the promoter fragments from the total genomic DNA were successfully amplified by PCR. These PCR products were purified and used to print an Arabidopsis promoter microarray. This initial promoter microarray was used to study the in vitro binding of the transcription factor HY5 to its promoter targets. A set of promoter fragments exhibited consistent and strong interaction with the HY5 protein in vitro, and computational analysis revealed that they were enriched with the HY5 consensus binding G-box motif. Thus, a promoter microarray can be a useful tool for identifying transcription factor binding sites at the genomic scale in higher plants.; Biochemistry & Molecular Biology; Plant Sciences; SCI(E); PubMed; 19; ARTICLE; 5; 683-699; 54
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/199985]
专题	生命科学学院
推荐引用方式 GB/T 7714	Gao, Y,Li, JM,Strickland, E,et al. An Arabidopsis promoter microarray and its initial usage in the identification of HY5 binding targets in vitro[J]. 植物分子生物学,2004.
APA	Gao, Y.,Li, JM.,Strickland, E.,Hua, SJ.,Zhao, HY.,...&Deng, XW.(2004).An Arabidopsis promoter microarray and its initial usage in the identification of HY5 binding targets in vitro.植物分子生物学.
MLA	Gao, Y,et al."An Arabidopsis promoter microarray and its initial usage in the identification of HY5 binding targets in vitro".植物分子生物学 (2004).