Expression cloning of functional receptor used by SARS coronavirus

doi:10.1016/j.bbrc.2004.01.076

CORC > 北京大学 > 生命科学学院

	Expression cloning of functional receptor used by SARS coronavirus
	Wang, PG ; Chen, J ; Zheng, AH ; Nie, YC ; Shi, XL ; Wang, W ; Wang, GW ; Luo, M ; Liu, HJ ; Tan, L ; Song, XJ ; Wang, Z ; Yin, XL ; Qu, XX ; Wang, XJ ; Qing, TT ; Ding, MX ; Deng, HK
刊名	生物化学与生物物理学研究通讯
	2004
关键词	expression cloning SARS-CoV ACE2 receptor FACS sorting TAIL-PCR S glycoprotein insertion activation LTR ACUTE RESPIRATORY SYNDROME TARGETED RECOMBINATION SPIKE GENE VIRUS GLYCOPROTEIN DETERMINANT GENOME
DOI	10.1016/j.bbrc.2004.01.076
英文摘要	We have expressed a series of truncated spike (S) glycoproteins of SARS-CoV and found that the N-terminus 14-502 residuals were sufficient to bind to SARS-CoV susceptible Vero E6 cells. With this soluble S protein fragment as an affinity ligand, we screened HeLa cells transduced with retroviral cDNA library from Vero E6 cells and obtained a HeLa cell clone which could bind with the S protein. This cell clone was susceptible to HIV/SARS pseudovirus infection and the presence of a functional receptor for S protein in this cell clone was confirmed by the cell-cell fusion assay. Further studies showed the susceptibility of this cell was due to the expression of endogenous angiotensin-converting enzyme 2 (ACE2) which was activated by inserted LTR from retroviral vector used for expression cloning. When human ACE2 cDNA was transduced into NIH3T3 cells, the ACE2 expressing NIH3T3 cells could be infected with HIV/SARS pseudovirus. These data clearly demonstrated that ACE2 was the functional receptor for SARS-CoV. (C) 2004 Elsevier Inc. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000189150300027&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; Biophysics; SCI(E); PubMed; 92; ARTICLE; 2; 439-444; 315
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/199663]
专题	生命科学学院
推荐引用方式 GB/T 7714	Wang, PG,Chen, J,Zheng, AH,et al. Expression cloning of functional receptor used by SARS coronavirus[J]. 生物化学与生物物理学研究通讯,2004.
APA	Wang, PG.,Chen, J.,Zheng, AH.,Nie, YC.,Shi, XL.,...&Deng, HK.(2004).Expression cloning of functional receptor used by SARS coronavirus.生物化学与生物物理学研究通讯.
MLA	Wang, PG,et al."Expression cloning of functional receptor used by SARS coronavirus".生物化学与生物物理学研究通讯 (2004).