HetR homodimer is a DNA-binding protein required for heterocyst   differentiation, and the DNA-binding activity is inhibited by PatS

doi:10.1073/pnas.0400429101

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	HetR homodimer is a DNA-binding protein required for heterocyst differentiation, and the DNA-binding activity is inhibited by PatS
	Huang, X ; Dong, YQ ; Zhao, JD
刊名	proceedings of the national academy of sciences of the united states of america
	2004
关键词	SP STRAIN PCC-7120 ANABAENA SP PCC-7120 PATTERN-FORMATION PCC 7120 ESCHERICHIA-COLI GENE-PRODUCT EXPRESSION CYANOBACTERIA NTCA ACTIVATION
DOI	10.1073/pnas.0400429101
英文摘要	HetR plays a key role in regulation of heterocyst differentiation. When the Cys-48 residue of the HetR from Anabaena sp. PCC 7120 was replaced with an Ala residue, the mutant HetR (HetR(C48A)) could not dimerize, indicating that HetR forms a homodimer through a disulfide bond. The Anabaena strain C48, containing the hetRc48a gene, could not produce HetR homodimer and failed to form heterocyst. We show that HetR is a DNA-binding protein and that its homodimerization is required for the DNA binding. HetR binds the promoter regions of hetR, hepA, and patS, suggesting a direct control of the expression of these genes by HetR. We present evidence that shows that the up-regulation of patS and hetR depends on DNA binding by HetR dimer. The pentapeptide RGSGR, which is present at the C terminus of PatS and blocks heterocyst formation, inhibits the DNA binding of HetR and prevents hetR up-regulation.; Multidisciplinary Sciences; SCI(E); PubMed; 101; ARTICLE; 14; 4848-4853; 101
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/199610]
专题	生命科学学院
推荐引用方式 GB/T 7714	Huang, X,Dong, YQ,Zhao, JD. HetR homodimer is a DNA-binding protein required for heterocyst differentiation, and the DNA-binding activity is inhibited by PatS[J]. proceedings of the national academy of sciences of the united states of america,2004.
APA	Huang, X,Dong, YQ,&Zhao, JD.(2004).HetR homodimer is a DNA-binding protein required for heterocyst differentiation, and the DNA-binding activity is inhibited by PatS.proceedings of the national academy of sciences of the united states of america.
MLA	Huang, X,et al."HetR homodimer is a DNA-binding protein required for heterocyst differentiation, and the DNA-binding activity is inhibited by PatS".proceedings of the national academy of sciences of the united states of america (2004).